摘要
为明确禽源α干扰素(IFN-α)对禽网状内皮组织增殖病病毒(REV)体内外增殖的抑制效果,本试验在细胞和动物水平上分别采用鸡胚成纤维(DF-1)细胞和海兰褐鸡作为模型,于REV接种前(预防)和接种后(治疗)分别添加不同浓度的禽IFN-α处理,分析禽IFN-α在体外和体内对REV增殖的影响。体外试验结果显示,与阳性对照组相比,禽IFN-α浓度为33 IU/mL的治疗组72 h细胞和上清中REV的增殖受到了显著抑制(P<0.05),禽IFN-α浓度为33 IU/mL的预防组72 h细胞中REV的增殖受到了显著抑制(P<0.05)。通过比较各组海兰褐鸡的平均体重、血液中REV阳性率和病毒载量、泄殖腔REV阳性率和排毒量、免疫器官指数和病毒载量系统评估禽IFN-α对REV体内增殖的影响,结果显示,与阳性对照组相比,禽IFN-α浓度为1 500 IU的治疗组海兰褐鸡21和28日龄时体重显著升高(P<0.05),14日龄时肝脏发育指数以及14和28日龄时的脾脏发育指数显著降低(P<0.05),14日龄时血液和脾脏中病毒载量显著降低(P<0.05),7和21日龄时泄殖腔排毒量显著降低(P<0.05)。本试验在体外细胞和体内动物2个层面均证实了禽IFN-α对REV增殖具有显著的抑制效果,这不仅为禽网状内皮组织增殖病的防控策略提供了新的思路和辅助手段,也为未来抗病毒药物的研发和应用提供了科学依据。
To determine the inhibitory effect of avian interferon-alpha(IFN-α)on the proliferation of reticuloendotheliosis virus(REV)in vivo and in vitro,this study used Douglas foster-1(DF-1)cells and Hailan Brown chickens as models.Different concentrations of avian IFN-αwere added before(prevention)and after(treatment)REV inoculation to assess its effect on REV proliferation.In vitro results showed that,compared with the Positive control group,the Treatment group with 33 IU/mL avian IFN-αexhibited significant inhibition of REV proliferation in cells and supernatant at 72 hours(P<0.05).Similarly,the Prevention group with 33 IU/mL avian IFN-αshowed significant inhibition of REV proliferation in cells at 72 hours(P<0.05).In vivo effects were evaluated by comparing the average body weight,blood REV positivity rate,viral load,cloacal virus shedding rate,immune organ index,and viral load in various groups of Hailan Brown chickens.The results indicated that,compared with the Positive control group,chickens in the Treatment group with 1500 IU of avian IFN-αhad significantly higher body weights at 21 and 28 days of age(P<0.05),and significantly lower liver development index at 14 days and spleen development index at 14 and 28 days(P<0.05).Additionally,viral load in blood and spleen at 14 days and cloacal virus shedding at 7 and 21 days were significantly reduced(P<0.05).This study confirmed that avian IFN-αsignificantly inhibits REV proliferation at both the cellular and animal levels.These findings not only offer new approaches for controlling reticuloendotheliosis but also provide a scientific basis for the development and application of future antiviral drugs.
作者
徐凤霞
孙万里
张亚文
常爽
王一新
赵鹏
XU Fengxia;SUN Wanli;ZHANG Yawen;CHANG Shuang;WANG Yixin;ZHAO Peng(College of Animal Science and Veterinary Medicine,Shandong Agricultural University,Tai'an 271018,China;Shandong Hekangyuan Biological Breeding Co.,Ltd.,Ji'nan 250001,China)
出处
《中国兽医杂志》
CAS
北大核心
2024年第10期10-17,共8页
Chinese Journal of Veterinary Medicine
基金
山东省重点研发计划(乡村振兴科技创新提振行动计划)(2022TZXD0019)。
