摘要
华木莲是木兰科的珍贵濒危树种,具有重要的潜在应用价值。本研究采用华木莲叶片为实验材料,通过单因素实验,探究华木莲叶片诱导愈伤组织的最佳消毒时长、最适宜激素浓度配比及最佳培养基。结果表明:(1)以0.1%的升汞溶液(HgCl_(2))作为消毒剂,消毒时长为8 min时,华木莲叶片诱导愈伤组织培养外植体污染率最低;(2)MS培养基为华木莲叶片组织培养的最佳基本培养基;(3)华木莲叶片诱导愈伤组织形成最适激素配比为MS+2.0 mg/L 6-BA+0.2 mg/L IBA。研究结果是对华木莲组织培养技术的经验总结,同时对华木莲的保护与利用有一定的实际意义。
Sinomanglietia glauca is a precious and endangered tree species in Magnoliaceae family,with important potential application value.In this study,the optimal disinfection time,optimal hormone concentration ratio and optimal culture medium to induce callus were explored with leaves of Sinomanglietia glauca through single factor experiment.The results showed that:(1)8 minutes was the optimal disinfection time,with 0.1%mercuric chloride solution(HgCl_(2))as disinfectant;(2)MS medium was the optimal basic medium for tissue culture of leaves of Sinomanglietia glauca;(3)The optimal hormone ratio for inducing callus formation in leaves was MS+2.0 mg/L 6-BA+0.2 mg/L IBA.These results are a summary of experience in tissue culture techniques of Sinomanglietia glauca,and have practical significance for its protection and utilization.
作者
余义亮
董霞
凌宇
程然然
YU Yiliang;DONG Xia;LING Yu;CHENG Ranran(Jingdezhen University,Jingdezhen 333400,China)
出处
《景德镇学院学报》
2024年第3期23-27,共5页
Journal of JingDeZhen University
基金
江西省教育厅科技项目(GJJ212808)
江西省教育厅科技项目(GJJ202806)。
关键词
华木莲
愈伤组织
消毒时长
激素浓度配比
培养基
Sinomanglietia glauca
disinfection duration
hormone concentration ratio
culture medium
