摘要
目的评价益胃汤加味对乙醇诱导胃溃疡模型大鼠的保护作用。方法SD大鼠随机分为对照组、模型组及益胃汤加味低、中、高剂量(0.8、1.2、1.7 g·kg^(-1))组,每组8只,各组均按剂量预给药30 d后,全部动物严格禁食24 h(不禁水)。除对照组外,其余大鼠每只均ig给予无水乙醇1.0 mL,1 h后牺牲动物取材。暴露完整胃,展开胃黏膜面拍照,对损伤进行评分;取部分胃组织进行苏木精-伊红(HE)染色;另取部分胃组织测定髓过氧化物酶(MPO)、总超氧化物歧化酶(SOD)、前列腺素E2(PGE2)、胃蛋白酶原Ⅰ(PGⅠ)、胃蛋白酶原Ⅱ(PGⅡ)水平;取血清检测其中血管内皮生长因子(VEGF)、白细胞介素-6(IL-6)、白细胞介素-1β(IL-1β)、肿瘤坏死因子-α(TNF-α)水平。此外,体外培养人胃黏膜上皮细胞(GES-1)及人脐静脉内皮细胞(HUVEC),通过MTT与划痕实验,评价益胃汤加味对增殖及迁移的作用。结果益胃汤加味可显著减轻胃黏膜损伤和降低胃黏膜病理评分(P<0.001);显著降低胃MPO活力并提高SOD活力(P<0.05、0.001);显著提高胃PGE2、PGⅠ、PGⅡ水平(P<0.05、0.001);显著降低血清TNF-α、IL-1β、IL-6水平(P<0.01、0.001);增加血清VEGF水平(P<0.05),促进GES-1和HUVEC细胞增殖与迁移(P<0.01、0.001)。结论益胃汤加味可通过抑制炎症因子分泌,缓解机体氧化应激,保护胃黏膜以及促进细胞增殖迁移等多种途径发挥对胃溃疡模型大鼠的保护作用。
Objective To evaluate the protective effect of Modified Yiwei Decoction on ethanol-induced gastric ulcer model rats.Methods SD rats were randomly divided into control group,model group and Modified Yiwei Decoction low-,medium-and highdose group(0.8,1.2 and 1.7 g·kg^(-1)),eight rats in each group.The drugs were given by ig administration before the model was established,once a day for 30 d,all the rats were strictly abstinence for 24 h(water freedom).Excluding rats in the control group,the rest of the rats were given 1 mL of absolute ethanol by ig administration to induce gastric ulcers.One hour later,all rats were sacrificed immediately.The gastric mucosal surface was unfolded to take pictures and the score of gastric damage was measured.HE staining of stomach sections were carried out to observe pathological changes.Myeloperoxidase(MPO),total superoxide dismutase(SOD),prostaglandin E2(PGE2),pepsinogen Ⅰ(PGⅠ),pepsinogen Ⅱ(PGⅡ),vascular endothelial growth factor(VEGF),interleukin-6(IL-6),interleukin-1β(IL-1β)and tumor necrosis factor-α(TNF-α)were detected.In addition,human gastric mucosal epithelial cells(GES-1)and human umbilical vein endothelial cells(HUVEC)were cultured in vitro,and the effects of Modified Yiwei Decoction on proliferation and migration were evaluated by MTT and scratch tests.Results Modified Yiwei Decoction can significantly reduce the damage of gastric mucosa and the pathological score of gastric mucosa(P<0.001).Gastric MPO activity was significantly decreased and serum SOD activity was increased(P<0.05,0.001).The contents of PGE2,PGI and PGII in stomach were significantly increased(P<0.05,0.001).The contents of TNF-α,IL-1β and IL-6 in serum were significantly decreased(P<0.01,0.001).And increased serum VEGF level(P<0.05)Promote proliferation and migration of GES-1 and HUVEC cells(P<0.01,0.001).Conclusion Modified Yiwei Decoction can inhibit the secretion of inflammatory factors,relieve oxidative stress,protect gastric mucosa,promote cell proliferation and migration,and play a protective role in gastric ulcer model rats.
作者
杨进
张珅
姚昌恒
李娜
吴韩
陈茜
谭玙晗
孙沐言
戴文玲
YANG Jin;ZHANG Shen;YAO Changheng;LI Na;WU Han;CHEN Qian;TAN Yuhan;SUN Muyan;DAI Wenling(China Pharmaceutical University,Nanjing 210000,China)
出处
《药物评价研究》
CAS
北大核心
2024年第9期2099-2106,共8页
Drug Evaluation Research
基金
中央高校基本科研业务费专项资金资助项目(2632023TD06)。
关键词
益胃汤加味
胃溃疡
抗炎
氧化应激
胃黏膜保护
增殖迁移
Modified Yiwei Decoction
gastric ulcer
anti-inflammatory
oxidative stress
gastric mucosal protection
proliferative migration
