摘要
目的 研究润燥止痒胶囊(RZZY)对湿疹大鼠肠道菌群及其代谢物短链脂肪酸(SCFA)的影响,以揭示RZZY对湿疹的改善作用及可能机制。方法 将大鼠分为正常组、模型组、地氯雷他定组(1.5 mg/kg)和RZZY低、高剂量组(0.6、1.2 g/kg)。除正常组外,其余各组大鼠采用2,4-二硝基氯苯溶液涂抹脱毛区域致敏以构建湿疹模型,并灌胃相应药物,每天1次,连续2周。给药完成后,检测大鼠皮肤厚度,并进行湿疹面积及严重度指数(EASI)评分和病理评分;基于16S rDNA测序分析大鼠肠道菌群结构;采用气相色谱-质谱联用仪检测大鼠粪便中SCFA(乙酸、丙酸和丁酸)含量;检测大鼠结肠和皮肤组织中信号转导及转录激活因子3(STAT3)的表达水平。结果 RZZY可明显减轻湿疹大鼠皮肤红肿、溃烂症状,显著减小/降低大鼠EASI、皮肤厚度、病理评分(P<0.05);显著升高湿疹大鼠肠道菌群ACE、Sobs和Shannon指数(P<0.05),促进大鼠肠道菌群向正常结构回调;提高湿疹大鼠肠道厚壁菌门和毛螺菌属、乳杆菌属、另枝菌属丰度,降低拟杆菌门/属丰度;显著升高湿疹大鼠粪便中丙酸、丁酸含量(P<0.05);显著下调结肠和皮肤组织中STAT3蛋白表达(P<0.05)。结论 RZZY可通过调节肠道菌群提高SCFA水平来抑制STAT3表达,从而发挥抗炎作用,进而改善湿疹大鼠的皮肤炎症。
OBJECTIVE To study the effect of Runzao zhiyang capsule(RZZY)on gut microbiota and its metabolites short-chain fatty acids(SCFA)in eczema rats,in order to reveal the improvement effect and possible mechanism of RZZY on eczema.METHODS The rats were divided into normal group,model group,desloratadine group(1.5 mg/kg),and RZZY low-dose and high-dose groups(0.6,1.2 g/kg).Except for the normal group,the eczema model was established in other groups by applying the 2,4-dinitrochlorobenzene solution to the hair removal area to sensitize it;they were given relevant drugs intragastrically,once a day,for consecutive 2 weeks.After medication,the skin thickness of rats was measured,and the eczema area and severity index(EASI)scoring and pathology scoring were performed;the structure of gut microbiota was analyzed by 16S rDNA sequencing.The contents of SCFA(including acetic acid,propionic acid,and butyric acid)in feces were determined by gas phase-mass spectrometry.The expressions of signal transduction and activator of transcription 3(STAT3)in the colon and skin were detected.RESULTS RZZY significantly alleviated skin swelling and ulceration and reduced EASI,skin thickness and pathological score in eczema rats(P<0.05).RZZY significantly increased ACE,Sobs and Shannon indexes(P<0.05)and promoted the microbiota of eczema rats to return to normal structure.RZZY increased the abundance of Firmicutes and Lachnospira,Lactobacillus and Alistipes,while decreased the abundance of Bacteroidetes and Bacteroides.RZZY significantly increased the contents of propionic acid and butyric acid in the feces of eczema rats(P<0.05),while significantly down-regulated the expressions of STAT3 protein in the colon and skin(P<0.05).CONCLUSIONS RZZY can improve SCFA levels by regulating gut microbiota,thereby inhibiting STAT3 expression,playing an anti-inflammatory role,and improving skin inflammation in eczema rats.
作者
胡银霞
陈梓钊
刘昌顺
郝勇
HU Yinxia;CHEN Zizhao;LIU Changshun;HAO Yong(Dept.of Gastroenterology,PLA General Hospital of Southern Theater Command,Guangzhou 510010,China;Clinical Medical College of Guangdong Pharmaceutical University,Guangzhou 510006,China;School of Traditional Chinese Medicine,Southern Medical University,Guangzhou 510515,China)
出处
《中国药房》
CAS
北大核心
2024年第19期2372-2377,共6页
China Pharmacy
基金
广东省基础与应用基础研究基金项目(No.2019A1515110600)。
关键词
润燥止痒胶囊
湿疹
肠道菌群
短链脂肪酸
Runzao zhiyang capsule
eczema
gut microbiota
short-chain fatty acid
