摘要
目的:观察针刺对类风湿关节炎(RA)小鼠肠道菌群的影响,探讨针刺治疗RA的作用机制。方法:C57BL/6J小鼠随机分为盐水组、模型组和针刺组,每组6只。采用右后足底注射完全弗氏佐剂复制RA模型。针刺组针刺“足三里”,每次30 min,每天1次,共7次。测量小鼠热刺激缩足潜伏期(PWTL)和足爪肿胀度。最后1次治疗结束后,收集各组小鼠粪便,采用16S RNA测序检测各组小鼠粪便中菌群的多样性及结构组成的变化。结果:与盐水组相比,模型组小鼠PWTL降低(P<0.01),足爪肿胀度升高(P<0.01);治疗后,与同时点模型组相比,针刺组针刺第1~7天PWTL升高(P<0.01),针刺第7天足爪肿胀度降低(P<0.01)。肠道菌群Alpha多样性分析,与盐水组比较,模型组小鼠菌落Chao1指数下降(P<0.05);与模型组比较,针刺组Chao1指数升高(P<0.05)。Beta多样性分析显示各组的肠道微生物群群落结构不同。在门水平上,与盐水组比较,模型组拟杆菌门的相对丰度减少(P<0.05),脱硫杆菌门的相对丰度增加(P<0.05);与模型组比较,针刺组放线菌门的相对丰度增加(P<0.05)。在属水平上,与盐水组比较,模型组埃希氏菌-志贺氏菌的丰度值增加(P<0.05);与模型组比较,针刺组埃希氏菌-志贺氏菌和肠杆菌属的丰度值降低(P<0.05),乳杆菌属丰度升高(P<0.01)。结论:针刺可明显缓解RA小鼠的关节炎性反应,其机制可能与调节RA小鼠肠道菌群多样性和结构组成有关。
Objective To observe the effect of acupuncture on gut microbiota in mice with rheumatoid arthritis(RA),so as to explore its mechanisms underlying improvement of RA.Methods Eighteen male C57BL/6J mice were randomly divided into saline,model and acupuncture groups,with 6 mice in each group.The RA model was established by injection of complete Freund's adjuvant(CFA,50μL)into the right hind paw.Acupuncture group received manual acupuncture stimulation of the bilateral“Zusanli”(ST36)for 30 min,once daily for 7 days.The paw withdrawal thermal latency(PWTL)was detected by using a thermal pain detector.After the last intervention,the mice’s feces were collected for extracting the feces DNA,and 16S RNA sequencing was used to detect and analyze the diversity and structural composition of intestinal flora.Results Compared with the saline group,the PWTL from day 0 to 7 after administration of CFA was significantly decreased(P<0.01),and the paw swelling volume from day 0 to 7 significantly increased in the model group(P<0.01).In comparison with the model group,the PWTL from day 1 to 7 was significantly increased(P<0.01),and the paw swelling volume on day 7 was strikingly decreased(P<0.01)in the acupuncture group.The Alpha diversity analysis of intestinal flora showed that the Chao1 index was markedly decreased in the model group compared with the saline group(P<0.05),and markedly increased in the acupuncture group compared with the model group(P<0.05).Beta diversity principal coordinate analysis(PCoA)displayed that the intestinal microbiota community structure of each group was different,and there were some overlapping areas,but they could be distinguished(P<0.01),suggesting that the species richness and diversity of RA mice were changed after acupuncture treatment.Compared with the saline group,the relative abundance of Bacteroidota was decreased(P<0.05),and that of Desulfobacterota and Escherichia-Shigella significantly increased(P<0.05)in the model group.In comparison with the model group,the relative abundance of Actinobacteria and Lactobacillus was considerably increased(P<0.05,P<0.01),while that of Escherichia-Shigella and Faecalibacterium strikingly decreased(P<0.05)in the acupuncture group.Conclusion Acupuncture treatment can significantly alleviate joint inflammation in RA mice,which may be related to its function in regulating the diversity and composition of gut microbiota.
作者
王宁
李媛
WANG Ning;LI Yuan(School of Acupuncture-moxibustion and Tuina&Health Preservation and Rehabilitation,Nanjing University of Chinese Medicine,Nanjing 210023,China)
出处
《针刺研究》
CAS
CSCD
北大核心
2024年第9期943-948,共6页
Acupuncture Research
基金
江苏省自然科学基金项目(No.BK20230453)
江苏省高校面上项目(No.22KJB360009)
江苏省卫健委医学科研项目指导性项目(No.Z2023032)。
关键词
针刺
类风湿关节炎
肠道菌群
Acupuncture
Rheumatoid arthritis
Gut microbiota
