摘要
为了评估在人工微颗粒饲料中添加扇贝肽(Scallop peptide)对大黄鱼(Larimichthys crocea)稚鱼的生长性能、摄食相关基因表达、消化酶活性、抗氧化力和炎症基因表达的影响。本实验以初始体质量(8.16±0.84)mg的大黄鱼稚鱼为研究对象,在饲料中分别添加0%、0.05%、0.1%和0.2%的扇贝肽,共配制4种等氮(粗蛋白54.15%)等脂(粗脂肪16.52%)的人工微颗粒饲料,进行为期30 d的摄食生长实验。研究表明,饲喂0.1%扇贝肽饲料稚鱼的FBL(终末体长)、FBW(终末体质量)和SGR(特定生长率)显著高于对照组(P<0.05)。在摄食相关基因表达方面,与对照组的相比,人工微颗粒饲料中添加0.1%扇贝肽可显著提高大黄鱼稚鱼的npy(神经肽Y)和ghrelin(饥饿素)的mRNA表达量,相应地,当稚鱼被饲喂含有0.1%扇贝肽的饲料时,稚鱼的trh(促甲状腺激素释放激素)和leptin(瘦素)的mRNA表达量显著降低(P<0.05)。在消化酶活性方面,饲喂0.1%扇贝肽饲料稚鱼的胰腺段和肠段的胰蛋白酶活性显著高于对照组,同时饲喂0.05%扇贝肽饲料的稚鱼胰腺段的胰蛋白活性也显著高于对照组(P<0.05)。在肠道发育方面,与对照组相比,在人工微颗粒饲料中添加0.1%扇贝肽可以显著提高稚鱼肠道刷状缘的LAP(亮氨酸氨基肽酶)和AKP(碱性磷酸酶活性)活性(P<0.05)。在抗氧化力方面,与对照组相比,人工微颗粒饲料中添加0.1%扇贝肽可显著提高稚鱼内脏团SOD(超氧化物歧化酶)活性,且降低稚鱼内脏团丙二醛(MDA)的含量(P<0.05)。饲喂0.1%扇贝肽饲料稚鱼的内脏团总一氧化氮合酶(T-NOS)活性和诱导型一氧化氮合酶(iNOS)活性显著高于对照组(P<0.05)。在炎症基因表达方面,与对照组相比,添加0.1%和0.2%扇贝肽饲料可显著降低稚鱼的il-1β(白介素-1β)、il-6(白介素-6)和il-8(白介素-8)的mRNA表达量(P<0.05)。综上所述,在人工微颗粒饲料中添加0.1%扇贝肽可以增强促食因子的mRNA表达量、抑制厌食因子的mRNA表达量、增强消化酶活性、提高抗氧化力及缓解炎症反应,最终提高大黄鱼稚鱼的生长性能。
A 30-day feeding trial was conducted to determine the effects of dietary scallop peptide supplementation on survival,growth performance,expression of feeding-related genes,activities of digestive enzymes,antioxidant capacity and expression of inflammatory factors of large yellow croaker larvae with an initial weight of(8.16±0.84)mg.Four 54.15%crude protein and 16.52%crude lipid diets were formulated with supplementation of 0%,0.05%,0.1%and 0.2%SP,respectively.Results indicated that larvae fed diets with 0.1%SP had higher specific growth rate than the control(P<0.05).Compared with the control,the mRNA expression of orexigenic factor genes including npy and ghrelin were significantly increased in larvae fed the diet with 0.1%SP,while the mRNA expression of anorexigenic factor genes including trh and leptin were significantly decreased in larvae fed the diet with 0.1%SP(P<0.05).The trypsin activity in larvae fed the diet with 0.1%SP was significantly higher than the control,while larvae fed the diet with 0.05%SP also had significantly higher trypsin activity than the control(P<0.05).The LAP and AKP in larvae fed the diet with 0.1%SP was significantly higher than the control(P<0.05).A clear increase of SOD was observed in larvae fed the diet with 0.1%SP compared with the control(P<0.05).Correspondingly,larvae fed the diet with 0.1%SP had significantly lower malondialdehyde(MDA)than the control.The total nitric oxide synthase(T-NOS)and inducible nitric oxide synthase(iNOS)of larvae fed the diet with 0.1%SP was significantly higher than the control.Moreover,the mRNA expression of interleukin-1β(il-1β),interleukin-6(il-6)and interleukin-8(il-8)(pro-inflammatory genes)in larvae fed diets with 0.1%and 0.2%SP were significantly lower than the control(P<0.05).In conclusion,the supplementation of 0.1%SP could enhance the expression of orexigenic factor genes,activities of digestive enzymes and antioxidant capacity,ultimately improving the growth performance of large yellow croaker larvae.
作者
艾庆辉
王云涛
张健敏
刘家辉
麦康森
Ai Qinghui;Wang Yuntao;Zhang Jianmin;Liu Jiahui;Mai Kangsen(The Key Laboratory of Aquaculture Nutrition and Feed of Agriculture and Rural Affairs,Ocean University of China,Qingdao 266003,China;The Key Laboratory of Mariculture(Ocean University of China),Ministry of Education,Qing-dao 266003,China)
出处
《中国海洋大学学报（自然科学版）》
CAS
CSCD
北大核心
2024年第10期145-156,共12页
Periodical of Ocean University of China
基金
现代农业产业技术体系项目(CARS-47-11)资助。
关键词
大黄鱼稚鱼
扇贝肽
摄食相关基因
消化酶活性
抗氧化力
large yellow croaker larvae
scallop peptide
feeding-related genes
digestive enzyme activity
antioxidant capacity
