摘要
为了筛选菜用甘薯的耐寒性鉴定指标,建立耐寒性数学评价模型,为菜用甘薯耐寒资源鉴定与评价提供依据,以5个菜用甘薯品种为材料,在12 h光照、12 h黑暗的条件下,以8.1~14.7℃(均温10.9℃)进行低温胁迫处理8 d,分别于处理后0、2、4、6、8 d测定甘薯叶片的SPAD值、膜脂过氧化相关指标、膜保护系统相关指标和细胞渗透调节物等,并运用主成分分析、隶属函数分析和逐步回归分析等方法对不同品种甘薯的耐寒性进行综合评价。结果显示:在低温胁迫条件下,不同品种甘薯的理化指标变化趋势存在一定差异;5个菜用甘薯品种的耐寒性表现为福菜薯23>福薯7-6>薯绿1号>C9>福菜薯18;菜用甘薯耐寒性评价数学模型PV(预测值)估计精度大于90%;脯氨酸含量、超氧化物歧化酶活性、过氧化物酶活性和可溶性蛋白含量可作为菜用甘薯耐寒性鉴定指标。
In order to screen cold resistance identification indicators for vegetable sweet potatoes,and establish a mathematical evaluation model for cold resistance to provide a basis for the identification and screening of cold resistant vegetable sweet potato resources,five varieties of vegetable sweet potato were subjected to conduct 8 days low temperature stress experiments under the conditions of 8.1-14.7℃,average temperature 10.9℃and 12h/12h day and night.Leaf SPAD value,membrane lipid peroxidation index,membrane protection system and cell osmotic regulator were determined at 0,2,4,6 and 8 d after treatment,respectively.Subsequently,principal component analysis,membership functions,and stepwise regression methods were used for comprehensive evaluation of cold resistance.The results showed that there were some differences in the change trend of physical and chemical indexes among different varieties under low temperature stress.The cold tolerance of five vegetable sweet potato varieties showed the trend of Fucaishu 23>Fushu 7-6>Shulyu No.1>C9>Fucaishu 18.The mathematical model PV(predicted value)of cold tolerance evaluation of vegetable sweet potato was established,and the estimation accuracy was more than 90%.Pro,SOD,POD and PS can be used as indicators for cold tolerance identification of vegetable sweet potatoes.
作者
钟坤权
梁薇
李想
肖晓
易镇邪
ZHONG Kunquan;LIANG Wei;LI Xiang;XIAO Xiao;YI Zhenxie(College of Agronomy,Hunan Agricultural University,Changsha,Hunan 410128,China)
出处
《作物研究》
2024年第4期278-286,共9页
Crop Research
基金
湖南省教育厅科研基金(20B302)。
关键词
菜用甘薯
低温胁迫
生理特性
耐寒性鉴定
vegetable sweet potato
low temperature stress
physiological characteristics
cold tolerance identification
