摘要
目的探讨长链非编码RNA核富含丰富的转录本1(long non-coding RNA nuclear-enriched abundant transcript1,LncRNA NEAT1),微小RNA(microRNA,miR)-424-5p对子宫内膜异位症(endometriosis,EM)并发不孕的诊断及对疾病分期的应用价值。方法将2021年10月~2023年10月在石家庄市妇幼保健院就诊的232例EM患者作为研究对象,其中120例为EM并发不孕患者作为不孕组,另外112例为单纯EM患者作为对照组,不孕组根据美国r-AFS分期标准将患者分为r-AFSⅠ~Ⅱ期(n=27)、r-AFSⅢ期(n=45)和r-AFSⅣ期(n=48);采用qRT-PCR法检测血清LncRNA NEAT1,miR-424-5p的表达水平;受试者工作特征(ROC)曲线分析血清LncRNA NEAT1,miR-424-5p诊断EM并发不孕的价值;多因素Logistic回归分析EM并发不孕患病的影响因素;在TargetScanHuman网站上进行LncRNA NEAT1靶向miR-424-5p的生物信息学分析;Pearson法分析EM并发不孕患者血清LncRNA NEAT1与miR-424-5p表达水平的相关性。结果不孕组和对照组血清LncRNA NEAT1(1.16±0.15 vs 1.02±0.13)、盆腔术史比例(17.50%vs 1.79%)、miR-424-5p(0.92±0.11 vs 1.04±0.12)表达水平比较,差异具有统计学意义(t/χ^(2)=7.753,16.018,7.974,均P<0.05);r-AFSⅠ~Ⅱ,Ⅲ,Ⅳ期患者血清LncRNA NEAT1(1.05±0.10,1.14±0.14,1.24±0.19)依次升高,miR-424-5p(0.99±0.12,0.93±0.11,0.87±0.10)表达水平依次降低,差异具统计学意义(F=13.528,10.910,均P<0.05);LncRNA NEAT1与miR-424-5p存在靶向关系,EM并发不孕患者血清LncRNA NEAT1与miR-424-5p表达水平呈负相关(r=-0.431,P<0.05)。血清LncRNA NEAT1,miR-424-5p及联合诊断EM并发不孕的AUC分别为0.782,0.719和0.835,二者联合诊断的AUC优于各自单独检测(Z=2.625,3.861,P=0.009,0.001);LncRNA NEAT1是EM并发不孕的危险因素,miR-424-5p是保护因素(P<0.05)。结论EM并发不孕患者血清LncRNA NEAT1表达水平升高,miR-424-5p表达水平下降,二者与疾病分期和不孕的发生有关。
Objective To explore the diagnostic value of long non-coding RNA nuclear-enriched abundant transcript 1(LncRNA NEAT1)and microRNA(miR)-424-5p in the diagnosis of endometriosis(EM)combined with infertility and its application value in disease staging.Methods A total of 232 EM patients who visited Shijiazhuang Maternal and Child Health Hospital from October 2021 to October 2023 were selected as the study subjects,120 patients with EM combined with infertility were used as the infertility group,and 112 patients with pure EM were regarded as the control group.The infertility group was separated into r-AFS stages I~II(n=27),r-AFS stage III(n=45),and r-AFS stage IV(n=48)according to the American r-AFS staging standards,and qRT-PCR method was applied to detect the expression levels of serum LncRNA NEAT1 and miR-424-5p.Receiver operating characteristic(ROC)curve was applied to analyze the value of serum LncRNA NEAT1 and miR-424-5p in diagnosing EM combined with infertility,and multivariate Logistic regression was applied to analyze the influencing factors of EM combined with infertility.Bioinformatics analysis of LncRNA NEAT1 targeting miR-424-5p was performed on the TargetScanHuman website.Pearson method was applied to analyze the correlation between serum LncRNA NEAT1 and miR-424-5p expression levels in patients with EM combined with infertility.Results Differences of serum LncRNA NEAT1(1.16±0.15 vs 1.02±0.13),pelvic surgery history ratio(17.50%vs 1.79%),miR-424-5p(0.92±0.11 vs 1.04±0.12)expression levels between infertility group and control group were significant,and the differences were significant(t=7.753,16.018,7.974,all P<0.05).Serum LncRNA NEAT1(1.05±0.10,1.14±0.14,1.24±0.19)increased and miR-424-5p(0.99±0.12,0.93±0.11,0.87±0.10)decreased in r-AFS stageⅠ~Ⅱ,Ⅲ,Ⅳpatients,and the differences were significant(F=13.528,10.910,all P<0.05).There was a targeting relationship between LncRNA NEAT1 and miR-424-5p,and there was a negative correlation between serum LncRNA NEAT1 and miR-424-5p expression levels in EM patients with infertility(r=-0.431,P<0.05).The AUCs of serum LncRNA NEAT1,miR-424-5p,and combined diagnosis for EM combined with infertility were 0.782,0.719 and 0.835,respectively,and the AUC of combined diagnosis was better than that of individual detection(Z=2.625,3.861,P=0.009,0.001).LncRNA NEAT1 was a risk factor for EM combined with infertility,while miR-424-5p was a protective factor(all P<0.05).Conclusion The expression level of serum LncRNA NEAT1 is increased and miR-424-5p is decreased in EM patients with infertility,both of which are related to the disease stage and the occurrence of infertility.
作者
孙晓清
崔照领
刘晓旭
SUN Xiaoqing;CUI Zhaoling;LIU Xiaoxu(the Six Department of Obstetrics,Shijiazhuang Maternal and Child Health Hospital,Shijiazhuang 050000,China;Department of Obstetrics and Gynecology,Shijiazhuang Maternal and Child Health Hospital,Shijiazhuang 050000,China;the Second Department of Obstetrics and Gynecology,Shijiazhuang Maternal and Child Health Hospital,Shijiazhuang 050000,China)
出处
《现代检验医学杂志》
CAS
2024年第5期54-57,74,共5页
Journal of Modern Laboratory Medicine
基金
河北省卫生健康委员会资助项目(20240735)。
