摘要
【目的】研究甜玉米基因Sugary1(Su1)序列,分析核苷酸多态性及检验,研究不同来源甜玉米中的Su1的等位变异位点,为甜玉米的品种改良和提高品质提供理论基础。【方法】以B73的Su1基因组序列为模板,对170份甜玉米自交系的Su1基因进行测序,研究玉米Su1基因序列的多态性。【结果】共有97个核苷酸位点发生变异,包含85个SNP位点,12个InDel位点。其中,位于编码区的SNP位点12个,InDel位点0个;位于非编码区的SNP位点85个,InDel位点12个。编码区中SNP位点同义突变有5个,非同义突变7个。自交系材料分为76个单倍型,该基因编码区序列将自交系材料分为47种单倍型。【结论】甜玉米Su1基因经历了明显的人工选择。
【Objective】The Sugary1(Su1)gene of sweet corn was analyzed by nucleotide polymorphism analysis and neutral test,and the allelic variation sites of Su1 in sweet corn from different sources were analyzed,which provided a theoretical basis for variety improvement and quality improvement of sweet corn.【Methods】In order to illustrate the sequence polymorphism of maize gene Su1,the Su1 gene in 170 sweet corn inbred lines was sequenced,using the genomic sequence of B73 as the reference.【Results】Analysis showed that 97 nucleotide sites were mutated,including 85 SNPs sites and 12 InDels locus.Among them,there were 12 SNPs loci and 0 InDel in the coding region,85 SNPs loci and 12 InDels in the non-coding region.There were 5 synonymous mutations at SNP site and 7 non-synonymous mutations in the coding region.The inbred lines were divided into 76 haplotypes according to the full-length sequence of Su 1 gene,and 47 haplotypes were divided into 47 haplotypes according to the coding region of the gene.【Conclusion】The Su1 gene had undergone obvious artificial selection.
作者
杨彩霞
顾炜
关媛
瞿静涛
党冬冬
吴鹏昊
郑洪建
YANG Caixia;GU Wei;GUAN Yuan;QU Jingtao;DANG Dongdong;WU Penghao;ZHENG Hongjian(College of Agronomy,Xinjiang Agricultural University,Urumqi,830052,China;Institute of Crop Breeding and Cultivation,Shanghai Academy of Agricultural Sciences/CIMMYT-China Special Maize Research Center,Shanghai 201403,China;Shanghai Special Maize Engineering Technology Research Center,Shanghai 201403,China)
出处
《新疆农业科学》
CAS
CSCD
北大核心
2024年第7期1605-1614,共10页
Xinjiang Agricultural Sciences
基金
上海市科学技术委员会科技计划项目“鲜食玉米营养、健康功能种质创新与新品种选育”(20392000400)
上海市科技兴农项目“糯玉米单倍体诱导系的创制及工程化育种应用”(2022-02-08-00-F01190)。
