摘要
为了探讨西门塔尔牛脆性X智力低下基因1(fragile X mental retardation 1,FMR1)的生物学功能,并为研究FMR1在西门塔尔牛屡配不孕中发挥的作用提供数据支持。实验以西门塔尔牛血液DNA为模板,通过PCR方法克隆FMR1基因完整的5′UTR序列;与NCBI提供的牛FMR1基因mRNA全序列进行比对;利用在线软件进行遗传分析并构建系统进化树,分析FMR1基因5′UTR端脆性;分析该基因编码蛋白的理化性质、亲疏水性、磷酸化位点、跨膜结构、信号肽、二级结构、三级结构和互作蛋白。结果表明:西门塔尔牛FMR1基因5′UTR序列与NCBI提供的牛FMR1基因序列基本一致,生物信息学分析得到牛FMR1基因最长开放阅读框1 710 bp,基因共编码569个氨基酸,分子量64 221.90 u,其编码蛋白是不稳定的亲水蛋白,没有明显的信号肽切割位点,并且不包括跨膜螺旋,二级结构预测表示其主要以α螺旋和无规卷曲形式为主。说明西门塔尔牛FMR1基因编码序列长度为40 254 bp,编码569个氨基酸,编码的蛋白质为亲水不稳定蛋白,与绵羊遗传距离最近。
To explore the biological function of fragile X mental retardation 1(FMR1)in Simmental cattle and to provide data support for the study of the role of FMR1 in the infertility of Simmental cattle,in this study,the complete 5′UTR sequence of FMR1 gene was cloned by PCR using Simmental cattle blood DNA as a template.It was compared with the full mRNA sequence of bovine FMR1 gene provided by NCBI.Online software was used for genetic analysis and phylogenetic tree construction to analyze the fragility of the 5′UTR end of FMR1 gene.The physicochemical properties,hydrophilicity,phosphorylation sites,transmembrane structure,signal peptide,secondary structure,tertiary structure and interacting proteins of the protein encoded by this gene were analyzed.The results showed that the 5′UTR sequence of Simmental cattle FMR1 gene was basically consistent with the sequence of cattle FMR1 gene provided by NCBI,and the longest open reading frame of bovine FMR1 gene was 1710 bp,the gene encoded 569 amino acids,and the molecular weight was 64221.90 u,and the encoded protein was an unstable hydrophilic protein,with no obvious signal peptide cleavage site,and did not include the transmembrane helix,and the secondary structure prediction indicated that it was mainly inα-helix and random coil form.The results indicated that the coding sequence of Simmental cattle FMR1 gene was 40254 bp and encoded 569 amino acids,and the encoded protein was a hydrophilic unstable protein,which showed the closest genetic distance from sheep.
作者
王选艺
孙亚伟
王俪颖
龙雨薇
叶彤
周瑜新
马雪连
李娜
赵红琼
姚刚
WANG Xuanyi;SUN Yawei;WANG Liying;LONG Yuwei;YE Tong;ZHOU Yuxin;MA Xuelian;LI Na;ZHAO Hongqiong;YAO Gang(College of Animal Medicine,Xinjiang Agricultural University,Urumqi 830052,Xinjiang)
出处
《浙江农业科学》
2024年第9期2190-2197,共8页
Journal of Zhejiang Agricultural Sciences
基金
新疆维吾尔自治区科技成果转化示范专项—乡村振兴产业发展科技行动项目(2022NC098)。
