摘要
肾类器官是利用人多能干细胞或组织来源的成体干细胞诱导分化形成主要包含肾小管结构的类器官,由于缺乏血管网络的支持,其组织结构不成熟和生长受限,如何实现肾类器官的血管化是该领域亟待解决的难题。目前,免疫缺陷动物移植、改变诱导分化方案、微流控芯片及调整细胞外基质和氧气浓度等方法可促进肾类器官血管化,为肾类器官科学研究及其临床应用提供了新的研究视角。
Kidney organoids are induced and differentiated from human pluripotent stem cells(PSCs)or adult stem cells(ASCs)derived from tissue sources,primarily composed of nephron struc-tures.However,due to the absence of a supporting vascular network,kidney organoids often exhibit immature tissue structures and limited growth.Therefore,vascularization in kidney organoids remains a pressing challenge in this field.Currently,the methods such as transplantation into immunodeficient animals,alterations in induced differentiation protocols,utilization of microfluidic chips,and manipula-tion of extracellular matrix and oxygen concentrations may facilitate vascularization of kidney organoids,which provides a new perspective for the scientific study and clinical application of kidney organoids.
作者
刘鹤
岳亮
吴成君
檀英霞
LIU He;YUE Liang;WU Chengjun;TAN Yingxia(School of Biomedical Engineering,Department of Medicine,Dalian University of Technology,Dalian 116000,China;Academy of Military Medical Sciences,Beijing 100850,China)
出处
《中国药理学与毒理学杂志》
CAS
北大核心
2024年第8期633-640,共8页
Chinese Journal of Pharmacology and Toxicology
关键词
肾类器官
血管化
微流控
异种移植
细胞外基质
kidney organoids
vascularization
microfluidics
xenotransplantation
extracellular matrix
