摘要
目的建立蜈蚣药材、饮片、标准汤剂与配方颗粒的高效液相色谱(HPLC)特征图谱,并测定其中次黄嘌呤、苯丙氨酸成分的含量,考察四者之间化学成分的传递与差异。方法采用Dikma Platisil ODS-C18(250 mm×4.6 mm,5μm)色谱柱,以甲醇和水为流动相梯度洗脱,流速为1 mL·min^(-1),柱温为25℃。以含量、特征图谱共有峰传递数及相似度为主要评价指标,分析其量值传递规律。结果18批蜈蚣药材、饮片、标准汤剂及3批配方颗粒特征图谱均有8个共有特征峰,与各自对照特征图谱相似度均大于0.90,指认出尿嘧啶、酪氨酸、次黄嘌呤、黄嘌呤、苯丙氨酸、肌苷、鸟苷、色氨酸8种成分,并测定其含量,未出现离散数据。结论蜈蚣药材、饮片、标准汤剂与配方颗粒的主要化学成分基本相同,HPLC特征图谱相关性良好,8种成分在测定范围内与峰面积线性关系良好。所建立质量评价模式能够反映蜈蚣4种形态多成分的整体面貌,为蜈蚣配方颗粒质量控制提供数据支撑。
Objective To establish the high performance liquid chromatography(HPLC)for the characteristics of Scolopendra and its decoction pieces,standard decoction and formula granules,and to determine the content of hypoxanthine and xanthine at the same time,and the transfer and difference in chemical components among the four.Methods Chromatography was performed on a Dikma Platisil ODS-C18 column(250 mm×4.6 mm,5μm).The methanol(A)and water(B)was used as the mobile phase with gradient elution.The flow rate was 1 mL·min^(-1),and the column temperature was 25℃.With the content,common peak transfer number and similarity of characteristic map as the main evaluation indexes,the quantitative value transfer was analyzed.Results There were 8 common characteristic peaks in the characteristic maps of 18 batches of Scolopendra and its decoction pieces,standard decoction and 3 batches of formula granules.The similarity between them and the control characteristic maps was greater than 0.90.Totally 8 components(uracil,tyrosine,hypoxanthine,xanthine,phenylalanine,inosine,guanosine,and tryptophan)were identified and their content was determined without discrete data.Conclusion The main chemical components in Scolopendra and its decoction pieces,standard decoction and formula granules are basically the same.The correlation of HPLC characteristic map is good,and the linearity of the 8 components is good within the determination concentration.The quality evaluation model can reflect the overall appearance of the four forms and components of Scolopendra,and provide data support for the quality control of the formula granules of Scolopendra.
作者
梁素仪
江斌
蔡小兵
李璐
张辉
谭沛
LIANG Su-yi;JIANG Bin;CAI Xiao-bing;LI Lu;ZHANG Hui;TAN Pei(China Resources Sanjiu Modern Chinese Medicine Pharmaceutical Co.,Ltd.,Huizhou Guangdong 516000;Anhui Key Laboratory of Traditional Chinese Medicine Formula Granules,Huaibei Anhui 235000;China Resources Sanjiu Medical&Pharmaceutical Co.,Ltd.,Shenzhen Guangdong 518000)
出处
《中南药学》
CAS
2024年第7期1903-1908,共6页
Central South Pharmacy
基金
深圳市科技计划项目(No.JSGG20191129093418578)。
关键词
蜈蚣
标准汤剂
配方颗粒
含量测定
特征图谱
Scolopendra
standard decoction
formula granule
content determination
characteristic chromatogram
