摘要
[目的]比较哺乳期健康犊牛和感染牛诺如病毒(Bovine norovirus,BNoV)的腹泻犊牛粪便菌群多样性和物种组成,初步揭示BNoV感染的腹泻犊牛粪便菌群特征。[方法]首先应用PCR和RT-PCR法对健康和腹泻犊牛粪便中的腹泻相关病原进行检测,然后采用16S rRNA扩增子测序技术对病原检测阴性的健康犊牛粪便(H组)和仅BNoV检测阳性的腹泻犊牛粪便(BNoV组)的微生物多样性、菌群组成和功能进行比较分析。[结果]与H组相比,BNoV组粪便菌群操作分类单元(OTUs)数量和Chao1指数显著升高(P<0.05),Shannon和Simpson指数极显著降低(P<0.01)。与H组相比,BNoV组粪便变形菌门、埃希-志贺属、丁酸球菌属丰度极显著升高(P<0.01),放线菌门、柯林斯菌属、巨球型菌属、厄氏菌属、双歧杆菌属、粪杆菌属等丰度极显著或显著降低(P<0.01;P<0.05)。双歧杆菌属、柯林斯菌属、巨球型菌属、厄氏菌属、粪杆菌属等在H组显著富集,而丁酸球菌属、埃希-志贺属、脆弱拟杆菌、梭菌属等在BNoV组显著富集。与H组相比,BNoV组粪便菌群胞内过程和信号传导、脂质代谢、新陈代谢、异生物质生物降解与代谢、萜类和聚酮类化合物代谢等显著或极显著上调(P<0.05;P<0.01),而膜转运、复制与修复、翻译、核苷酸代谢、遗传信息处理等显著或极显著下调(P<0.05;P<0.01)。[结论]感染BNoV的腹泻犊牛粪便菌群丰富度、多样性、物种组成和功能与健康犊牛显著不同,提示BNoV感染犊牛处于肠道菌群紊乱状态。
[Objective]In order to determine the characteristics of fecal microbiota of diarrheal calves infected with Bovine norovirus(BNoV),this study was conducted to compare the diversity and species composition of fecal microbiota between healthy calves and diarrheal calves with BNoV infection.[Method]Firstly,PCR and RT-PCR were conducted to detect diarrhea-associated pathogens in the feces of healthy and diarrheal calves.Then 16S rRNA amplicon sequencing technology was performed to compare the diversity,composition and function of microbiota in the feces of healthy calves with negative pathogen detection(group H)and those of diarrheal calves with positive BNoV detection only(group BNoV).[Result]Compared with group H,the OTUs and Chao1 index of fecal microbiota were significantly increased(P<0.05),the Shannon and Simpson indexes were decreased extremely significantly(P<0.01)in group BNoV.Compared with group H,the abundance of Proteobacteria,Escherich-Shigella and Butyricococcus was extremely significantly increased(P<0.01)in group BNoV.The abundance of Actinomycetes,Collinsella,Megasphaera,Olsenella,Bifidobacterium and Faecalibacterium was extremely significantly or significantly reduced(P<0.01 or P<0.05)in BNoV group.Bifidobacterium,Collinsella,Megasphaera,Olsenella and Faecalibacterium were significantly enriched in group H,while Butyricococcus,Escherichia-Shigella,Bacteroides fragilis and Clostridium were significantly enriched in group BNoV.Compared with group H,cellular processes and signaling,lipid metabolism,metabolism,xenolism biodegradation and metabolism,metabolism of terpenoids and polyketides of fecal microbiota were significantly or extremely significantly up-regulated(P<0.05 or P<0.01)in group BNoV,while membrane transport,replication and repair,translation,nucleotide metabolism,genetic information processing were significantly or extremely significantly down-regulated(P<0.05 or P<0.01).[Conclusion]The richness,diversity,species composition and function of fecal microbiota in calves with diarrhea associated with BNoV infection were significantly different from those of healthy calves,suggesting that the intestinal microbiota of BNoV infected calves was disturbed.
作者
赵清梅
崔省委
郭仕辉
余永涛
梁泰宇
李欢语
ZHAO Qingmei;CUI Shengwei;GUO Shihui;YU Yongtao;LIANG Taiyu;LI Huanyu(College of Biological Science and Engineering,North Minzu University,Yinchuan 750021,China;College of Animal Science and Technology,Ningxia University,Yinchuan 750021,China;Key Laboratory of Ruminant Molecular and Cellular Breeding in Ningxia,Yinchuan 750021,China)
出处
《中国畜牧兽医》
CAS
CSCD
北大核心
2024年第8期3484-3494,共11页
China Animal Husbandry & Veterinary Medicine
基金
宁夏自然科学基金项目(2022AAC03231)
宁夏回族自治区重点研发计划项目(2021BEF02028、2021BEF01001)。
