摘要
本研究比较分析了4个不同等级(特一级、特二级、一级、二级)霍山黄芽的主要生化成分和抗氧化活性差异,并选取特一级霍山黄芽,基于棕榈酸诱导的小鼠正常肝细胞AML-12(alpha mouse liver 12)、小鼠神经小胶质细胞BV-2、人肾皮质近曲小管上皮细胞HK-2(human kidney-2)、人胃腺癌细胞(human gastric adenocarcinoma,AGS)细胞模型,研究霍山黄芽提取物(Huoshanhuangya tea extract, HTE)对脂质积累和促炎细胞因子的影响。结果表明:特一级霍山黄芽的可溶性糖质量分数最低,咖啡碱质量分数、儿茶素类质量分数与游离氨基酸总量最高,且抗氧化活性最强;一定质量浓度的HTE处理后,棕榈酸诱导的AML-12、BV-2、HK-2、AGS细胞的细胞活力显著增加,棕榈酸诱导的脂质积累得到改善,棕榈酸诱导的AML-12、BV-2、HK-2细胞中肿瘤坏死因子-α(tumor necrosis factor-α, TNF-α)的释放水平明显降低。进一步探究其作用机制发现,HTE使BV-2细胞中炎症信号通路Toll样受体4(Toll-like receptor 4, TLR4)和核因子-κB RelA(nuclear factor-κB RelA, NF-κB p65)基因的表达下调,表明其可能通过抑制TLR4/NF-κB信号通路的活化来缓解棕榈酸诱导的BV-2细胞炎症损伤。
This study aimed to compare and analyze the major biochemical components and antioxidant activities of four different grades(special-grade 1,special-grade 2,grade 1 and grade 2)of Huoshanhuangya tea.Special-grade 1 Huoshanhuangya tea was chosen to study the effects of Huoshanhuangya tea extract(HTE)on lipid accumulation and proinflammatory cytokines via palmitic acid-induced alpha mouse liver 12(AML-12),BV-2,human kidney-2(HK-2),and human gastric adenocarcinoma(AGS)cells.The results revealed that the special-grade 1 Huoshanhuangya tea had the lowest soluble sugar content,the highest caffeine content,catechins content and total free amino acid content,and the strongest antioxidant activity.After treatment with HTE at a certain concentration,the viability of palmitic acid-induced AML-12,BV-2,HK-2 and AGS cells significantly increased;the lipid accumulation induced by palmitic acid was inhibited;and the release levels of proinflammatory cytokines such as tumor necrosis factor-α(TNF-α)in palmitic acid-induced AML-12,BV-2 and HK-2 cells were significantly decreased.To further explore its mechanism of action,it was found that HTE down-regulated the expression of Toll-like receptor 4(TLR4)and nuclear factor-κB RelA(NF-κB p65)in the inflammatory signaling pathway of BV-2 cells,suggesting that HTE may relieve the palmitic acid-induced inflammatory damage of BV-2 cells by inhibiting the activation of the TLR4/NF-κB signaling pathway.
作者
左欣欣
陈蔚
丁乐佳
施羽萱
周继红
刘仲华
王岳飞
ZUO Xinxin;CHEN Wei;DING Lejia;SHI Yuxuan;ZHOU Jihong;LIU Zhonghua;WANG Yuefei(Tea Research Institute,College of Agriculture and Biotechnology,Zhejiang University,Hangzhou 310058,Zhejiang,China;Key Lab of Tea Science,Ministry of Education/National Research Center of Engineering Technology for Utilization Ingredients from Botanicals/Co-Innovation Center of Education Ministry for Utilization of Botanical Functional Ingredients,Hunan Agricultural University,Changsha 410128,Hunan,China)
出处
《浙江大学学报（农业与生命科学版）》
CAS
CSCD
北大核心
2024年第4期633-645,共13页
Journal of Zhejiang University:Agriculture and Life Sciences
基金
国家自然科学基金区域创新发展联合基金项目(U19A2034)。
关键词
黄茶
饱和脂肪酸
抗氧化活性
脂质积累
促炎细胞因子
yellow tea
saturated fatty acid
antioxidant activity
lipid accumulation
proinflammatory cytokines
