摘要
目的 探索铁死亡驱动因子细胞因子信号转导抑制蛋白1(SOCS1)与抑制因子铁蛋白重链1(FTH1)在鼻咽癌中的表达水平及电离辐射对其的影响。方法 分析来自GEO数据库的鼻咽癌数据集GSE53819(18个鼻咽癌样本和18份鼻咽正常组织样本)和GSE12452(31个鼻咽癌样本和10份鼻咽正常组织样本)中SOCS1与FTH1的表达水平,并联合GSE62328(30个鼻咽癌样本)分析不同肿瘤分期、淋巴结转移状态与原发-复发类型中SOCS1和FTH1表达水平;利用GSE48501数据集中放射抵抗性鼻咽癌CNE2-IR细胞和放射敏感性鼻咽癌CNE2细胞中m RNA表达数据,分析SOCS1与FTH1在放射抵抗性和放射敏感性鼻咽癌细胞株中的表达水平;以经2 Gy电离辐射照射后的鼻咽癌C666-1细胞为实验组,0 Gy电离辐射照射的鼻咽癌C666-1细胞为对照组,应用RT-q PCR法分别检测其中SOCS1与FTH1的表达水平。结果 GEO数据库分析显示,鼻咽癌组织中SOCS1与FTH1表达水平高于正常鼻咽组织(P<0.05)。与伴淋巴结转移的鼻咽癌样本比较,无淋巴结转移的鼻咽癌样本中SOCS1与FTH1表达水平较高(P<0.05)。SOCS1与FTH1在放射抵抗性和放射敏感性鼻咽癌细胞株中的表达水平比较,差异无统计学意义(P>0.05)。RT-PCR结果提示,实验组C666-1细胞中SOCS1表达水平高于对照组,FTH1表达水平低于对照组(P<0.05)。结论 铁死亡驱动因子SOCS1与抑制因子FTH1在鼻咽癌中差异表达且可作为鼻咽癌放射治疗潜在靶点。
Objective To explore the expression levels of iron death driver suppressors of cytokine signaling1(SOCS1)and repressor ferritin heavy chalin 1(FTH1)in nasopharyngeal carcinoma and the effects of ionizing radiation on them.Methods The expression levels of SOCS1 and FTH1 in the nasopharyngeal carcinoma datasets GSE53819(18 nasopharyngeal carcinoma samples and 18 nasopharyngeal normal tissue samples)and GSE12452(31 nasopharyngeal carcinoma samples and ten nasopharyngeal normal tissue samples)from the GEO database were analyzed,and the relationship between clinical characteristics such as different tumor stages and lymphatic metastasis status and primary-recurrent type and the levels of SOCS1 and FTH1 expression were analyzed in combination with GSE62328(30 nasopharyngeal carcinoma samples);the mRNA expression data in radioresistant nasopharyngeal carcinoma CNE2-IR cells and radiosensitive nasopharyngeal carcinoma CNE2 cells in the GSE48501 dataset were utilized to analyze the expression levels of SOCS1 versus FTH1 in the radioresistant and radiosensitive nasopharyngeal carcinoma cell lines;nasopharyngeal carcinoma C666-1 cells irradiated by 2 Gy of ionizing radiation were used as the experimental group,and nasopharyngeal carcinoma C666-1 cells irradiated by 0 Gy of ionizing radiation were used as the control group,and RT-qPCR was applied to detect the expression levels of SOCS1 and FTH1 in them,respectively.Results GEO database analysis showed that the expression levels of SOCS1 and FTH1 were higher in nasopharyngeal cancer tissues than those in normal nasopharyngeal tissues(P<0.05).Compared with nasopharyngeal cancer samples with lymph node metastasis,the expression levels of SOCS1 and FTH1 were higher in nasopharyngeal cancer samples without lymph node metastasis(P<0.05).There was not significant difference in the expression levels of SOCS1 and FTH1 in radioresistant and radiosensitive nasopharyngeal cancer cell lines(P>0.05).The results of real-time fluorescence quantitative PCR suggested that the expression level of SOCS1 in C666-1 cells of the experimental group was higher than that of the control group,and the expression level of FTH1 was lower than that of the control group(P<0.05).Conclusion Ferroptosis driver SOCS1 and suppressor FTH1 are differentially expressed in nasopharyngeal carcinoma and may serve as potential targets for nasopharyngeal carcinoma radiotherapy.
作者
金仙槐
曾宪琳
龙金华
JIN Xianhuai;ZENG Xianlin;LONG Jinhua(Department of Oncology,Affiliated Hospital of Guizhou Medical University,Guizhou Province,Guiyang550004,China;Department of Oncology,Affiliated Cancer Hospital of Guizhou Medical University,Guizhou Province,Guiyang550004,China;Department of Oncology,School of Clinical Medicine,Guizhou Medical University,Guizhou Province,Guiyang550004,China;School of Biology and Engineering,Guizhou Medical University,Guizhou Province,Guiyang550004,China)
出处
《中国医药导报》
CAS
2024年第19期106-111,共6页
China Medical Herald
基金
国家自然科学基金资助项目(82060555)。
关键词
细胞因子信号抑制蛋白1
铁蛋白重链1
鼻咽癌
铁死亡
放射治疗
Suppressors of cytokine signaling1
Ferritin light heavy chain 1
Nasopharyngeal Carcinoma
Iron death
Radiotherapy
