摘要
目的:探讨白芍总苷(TGP)调节磷脂酰肌醇3-激酶(PI3K)/蛋白激酶B(Akt)信号通路对大鼠原发性痛经(PDM)的改善作用。方法:将雌性SD大鼠采用随机数字表法分为正常对照组(Nor组)、PDM模型组(PDM组)、低剂量TGP组(TGP-L组)、高剂量TGP组(TGP-H组)和高剂量TGP+PI3K激活剂740Y-P组(TGP-H+740Y-P组),每组12只。除Nor组外,其余各组大鼠采用苯甲酸雌二醇联合缩宫素制备大鼠PDM模型,并分别用50、100 mg/kg TGP干预TGP-L组、TGP-H组大鼠,TGP-H+740Y-P组大鼠另需10 mg/kg 740Y-P干预。末次给药后观察大鼠的扭体反应,记录扭体次数和潜伏期,行扭体反应评分。HE染色观察大鼠子宫组织的病理学变化,行病理学评分。ELISA法检测大鼠子宫组织前列腺素F2α(PGF2α)、前列腺素E2(PGE2)水平以及血清β-内啡肽(β-EP)水平。免疫印迹法测量子宫组织PI3K、Akt表达及其磷酸化水平。结果:与Nor组比较,PDM组子宫病理损伤严重,子宫组织病理学评分、PGF2α以及p-PI3K、p-Akt水平升高,子宫组织PGE2及血清β-EP水平降低(均P<0.05)。与PDM组比较,TGP-L组、TGP-H组大鼠扭体反应潜伏期延长,扭体反应次数减少,扭体反应评分降低,子宫组织病理损伤减轻,子宫组织病理学评分、PGF2α以及p-PI3K、p-Akt水平降低,子宫组织PGE2及血清β-EP水平升高(均P<0.05)。高剂量TGP对大鼠PDM的改善作用更显著,而740Y-P减弱了TGP对大鼠PDM的改善作用。结论:TGP可能通过抑制PI3K/Akt信号通路改善大鼠PDM。
Objective:To investigate the improvement effect of total glucosides of paeony(TGP)on primary dysmenorrhea(PDM)in rats by regulating the phosphatidylinositol 3-kinase(PI3K)/protein kinase B(Akt)signaling pathway.Methods:Female SD rats were divided into the normal control group(Nor group),PDM model group(PDM group),low-dose TGP group(TGP-L group),high-dose TGP group(TGP-H group)and high-dose TGP+PI3K activator 740Y-P group(TGP-H+740Y-P group)according to random number table method,with 12 rats in each group.Except for the Nor group,rats in other groups were used to prepare rat PDM models using estradiol benzoate combined with oxytocin,and the rats in TGP-L group and TGP-H group were intervened with 50 mg/kg and 100 mg/kg of TGP,respectively.Rats in TGP-H+740Y-P group were additionally intervened with 10 mg/kg of 740Y-P.The torsion reaction of rats was observed after the last administration,and the number of torsion reaction and incubation period were recorded,and the torsion reaction score was performed.HE staining was applied to observe the pathological changes of uterine tissue,and pathological score was performed.ELISA method was applied to detect the levels of prostaglandin F2α(PGF2α),prostaglandin E2(PGE2)in uterine tissue,andβ-endorphin(β-EP)in serum.The expression and phosphorylation levels of PI3K and Akt in uterine tissue were measured by Western blot.Results:Compared with Nor group,the pathological injury of uterus in PDM group were serious,the pathological score,the levels of PGF2α,p-PI3K and p-Akt in uterine tissue were increased,while the level of PGE2 in uterine tissue andβ-EP in serum were decreased(all P<0.05).Compared with PDM group,TGP-L group and TGP-H group prolonged the incubation period of torsion reaction,reduced the number of torsion reaction,decreased incubation period score,alleviated pathological injury of uterine tissue,and pathological score,the levels of PGF2α,p-PI3K and p-Akt in uterine tissue were decreased,the level of PGE2 in uterine tissue andβ-EP in serum were increased(all P<0.05).High dose TGP improved PDM more significantly in rats,and 740Y-P weakened the improvement of TGP on PDM in rats.Conclusion:TGP may improve PDM in rats by inhibiting PI3K/Akt signaling pathway.
作者
戴亦娴
张晓鸣
华丰
刘产明
朱月琴
蒋婷
周甜
吴栋才
DAI Yixian;ZHANG Xiaoming;HUA Feng;LIU Chanming;ZHU Yueqin;JIANG Ting;ZHOU Tian;WU Dongcai(Department of Pharmacy,Changzhou Hospital of Traditional Chinese Medicine,Changzhou 213000,China)
出处
《陕西医学杂志》
CAS
2024年第8期1021-1025,共5页
Shaanxi Medical Journal
基金
国家自然科学基金资助项目(61970225)
江苏省中医药管理局项目(202232)
江苏省常州市科技计划项目(CJ20229012)。
关键词
原发性痛经
白芍总苷
磷脂酰肌醇3-激酶
蛋白激酶B
大鼠
Primary dysmenorrhea
Total glucosides of paeony
Phosphatidylinositol 3-kinase
Protein kinase B
Rats
