摘要
目的基于p38MAPK信号通路探讨火针治疗膝骨关节炎(KOA)软骨退变的作用机制。方法30只SPF级雄性SD大鼠随机分为空白组、模型组和火针组,每组10只。模型组和火针组采用膝关节腔注射木瓜蛋白酶法建立KOA模型。火针组于“内膝眼”“犊鼻”予火针干预,模型组和空白组仅固定、不干预,每3d一次,连续4周。HE染色和番红O-固绿染色观察大鼠膝关节软骨组织形态,TUNEL染色检测软骨细胞凋亡指数,免疫组化染色检测膝关节软骨组织p-p38和Fas蛋白表达,qPCR检测p38和FasmRNA表达。结果与空白组比较,模型组大鼠膝关节软骨组织表面凹凸不平,软骨细胞排列不规则、分布不均匀,基质红染变浅,潮线不清晰,改良Mankin's评分显著升高(P<0.001),软骨细胞凋亡指数显著升高(P<0.01),膝关节软骨组织p-p38、Fas蛋白表达显著升高(P<0.01),p38、Fas mRNA表达显著升高(P<0.001);与模型组比较,火针组大鼠膝关节软骨组织表面相对平整,软骨细胞排列大致正常,基质染色轻度变浅,改良Mankin's评分显著降低(P<0.001),软骨细胞凋亡指数显著下降(P<0.01),膝关节软骨组织p-p38、Fas蛋白表达显著降低(P<0.01),p38、FasmRNA表达显著下降(P<0.001)。结论火针可能通过抑制p38MAPK信号通路激活减少膝关节软骨细胞凋亡,进而延缓KOA进展。
Objective To explore the mechanism of fire needle in treating cartilage degeneration in knee osteoarthritis(KOA)based on p38MAPK signaling pathway.Methods Totally 30 SPF grade male SD rats were randomly divided into blank group,model group and fire needle group,with 10 rats in each group.The model group and fire needle group established KOA models by injecting papain into the knee joint cavity.The fire needle group received fire needle intervention in“Neixiyan”and“Dubi”,while the model group and blank group only received fixation and no intervention,once every 3 days,for 4 consecutive weeks.Morphological changes in the knee joint cartilage were observed using HE staining and safranine O-solid green staining,TUNEL staining was used to detect the apoptosis index of chondrocytes,immunohistochemical staining was used to detect the expressions of p-p38 and Fas protein,qPCR was used to detect the expressions of p38 and Fas mRNA.Results Compared with the blank group,the surface of the knee joint cartilage in the model group rats was uneven,with irregular and uneven arrangement of chondrocytes,lighter matrix red staining,and unclear tidal lines,the improved Mankin’s score significantly increased(P<0.001),the apoptosis index of chondrocytes significantly increased(P<0.01),the expressions of p-p38 and Fas protein in knee joint cartilage tissue significantly increased(P<0.01),and the expressions of p38 and Fas mRNA significantly increased(P<0.001).Compared with the model group,the surface of the knee joint cartilage of the fire needle group rats was relatively flat,the arrangement of chondrocytes was generally normal,and the matrix staining was slightly lighter,the improved Mankin’s score significantly decreased(P<0.001),the apoptosis index of chondrocytes significantly decreased(P<0.01),the expressions of p-p38 and Fas protein in knee joint cartilage tissue significantly decreased(P<0.01),and the expressions of p38 and Fas mRNA significantly decreased(P<0.001).Conclusion Fire needle may delay the progression of KOA by inhibiting the activation of p38MAPK signaling pathway and reducing chondrocyte apoptosis of knee joint.
作者
万甜
肖阳
石欣悦
洪昆达
WAN Tian;XIAO Yang;SHI Xinyue;HONG Kunda(The Second Affiliated Hospital of Fujian Medical University,Quanzhou 362000,China;College of Acupuncture and Moxibustion,Fujian University of Traditional Chinese Medicine,Fuzhou 350122,China)
出处
《中国中医药信息杂志》
CAS
CSCD
2024年第8期70-75,共6页
Chinese Journal of Information on Traditional Chinese Medicine
基金
福建省卫健委中青年骨干人才项目(2020GGA055)
福建省自然科学基金面上项目(2023J01732)。
