摘要
关注牛奶安全这一国计民生问题,将分子印迹前沿技术引入本科实验课堂,设计了一个综合实验。融合分子印迹、蛋白质排阻、磁性分离等技术制备得到磁性分子印迹聚合物,考察其性能,并以其作为固相萃取吸附剂,结合高效液相色谱,建立直接选择性检测牛奶样品中痕量四环素的新方法。本实验涉及材料化学、分析化学、仪器分析、食品化学等多学科知识,培养学生的科学素养、综合解决复杂问题的能力和创新性思维。印迹材料的设计和磁性分离过程富有创新性和趣味性,整个实验过程成本低廉、易操作、安全性高。通过本实验,学生能够了解分子印迹前沿技术,学习新检测方法的建立,巩固实验基础操作,提高数据采集和分析能力,加深对组分-性能-应用关系的理解。
This experiment addresses the pressing issue of milk safety,integrating cutting-edge molecular imprinting technology into undergraduate laboratory classes.A comprehensive experiment is designed,combining molecular imprinting,protein exclusion,and magnetic separation techniques to synthesize lightweight magnetic molecularly imprinted polymers.The polymers’performance is evaluated,and they are utilized as solid-phase extraction adsorbents in conjunction with high-performance liquid chromatography to establish a novel method for the direct and selective detection of trace tetracycline in milk samples.This interdisciplinary study spans materials chemistry,analytical chemistry,instrumental chemistry,and food chemistry,fostering students’scientific literacy,problem-solving abilities,and innovative thinking.The innovative design of the imprinting material and magnetic separation process ensures cost-effectiveness,ease of operation,and high safety throughout the experiment.Through this hands-on experience,students gain insight into molecular imprinting technology,learn new detection methods,refine basic laboratory skills,enhance data collection and analysis capabilities,and deepen their understanding of the interplay between components,performance,and application.
作者
张军杰
王悦
吴秋含
沈如泉
刘涵
段新华
Junjie Zhang;Yue Wang;Qiuhan Wu;Ruquan Shen;Han Liu;Xinhua Duan(School of Chemistry,Xi’an Jiaotong University,Xi’an 710049,China)
出处
《大学化学》
CAS
2024年第5期251-257,共7页
University Chemistry
基金
2022年西安交通大学本科教学改革研究项目(2203Z)。
关键词
分子印迹聚合物
牛奶
四环素
综合化学实验
Molecularly imprinted polymer
Milk
Tetracycline
Comprehensive chemistry experiment
