摘要
【目的】探究羽衣甘蓝裂叶表型形成的分子机制,研究BoLMI基因对裂叶形成的作用。【方法】以裂叶羽衣甘蓝DH系为试验材料,利用同源克隆技术,成功获取基因完整的cDNA序列,将其命名为BoLMI。【结果】序列分析发现,BoLMI基因全长531个碱基对,共编码176个氨基酸,其蛋白分子量为20 789.35 Da,理论等电点为7.24。根据Pfam保守结构域分析,BoLMI蛋白包含homeobox保守结构域。系统发育分析结果显示,羽衣甘蓝的BoLMI基因与甘蓝型油菜以及结球甘蓝的BoLMI基因同属于一个分支,亲缘关系较近。BoLMI蛋白是位于细胞核内的可溶性蛋白,包含1个跨膜结构域,无信号肽序列。qRT-PCR分析表明,BoLMI基因在裂叶羽衣甘蓝的幼苗期表达水平较高,在莲座期表达水平较低;在羽衣甘蓝莲座期,裂叶品种不同部位叶片BoLMI基因的表达水平均高于圆叶品种,其中在第1片叶的表达量最高。裂叶品种和圆叶品种不同叶片BoLMI基因的表达量差异显著。【结论】推测BoLMI基因在羽衣甘蓝裂叶形成中起重要作用,为加速羽衣甘蓝叶形育种提供了理论基础。
[Objective]The study aimed to investigate the molecular mechanism of phenotype formation in kale cleavage leaves,and to investigate the role of BoLMI gene in split leaf formation.[Method]Using the DH line of split leaf kale as the test material,the complete cDNA se-quence of the gene was successfully obtained using homologous cloning technology,and it was named BoLMI.[Result]The sequence analysis showed that the full-length of BoLMI was 531 base pairs and encoded 176 amino acids.The protein had a molecular weight of 20789.35 Da and a theoretical isoelectric point of 7.24.According to Pfam conserved domain analysis,BoLMI protein contained a homeobox conserved domain.Phylogenetic tree analysis showed that the BoLMI of kale belonged to the same branch as that of Brassica napus and B.oleracea,and the genetic relationship was relatively close.BoLMI protein was a soluble protein located in the nucleus,containing one transmembrane domain and no signal peptide sequence.The qRT-PCR analysis showed that the expression level of BoLMI was higher that in the seedling stage of split leaf kale and lower that in the rosette stage.During the lotus stage of kale,the expression levels in different parts of the leaves of cracked leaf varieties were higher than those of round leaf varieties,with the highest expression level in the first leaf.There was a significant difference in expression levels between cracked leaf varieties and round leaf varieties.[Conclusion]It is speculated that the BoLMI gene plays an important role in the formation of split leaves in kale,providing a theoretical basis for accelerating kale leaf shape breeding.
作者
姚悦梅
任杰
山溪
戴忠良
张振超
YAO Yue-mei;REN Jie;SHAN Xi;DAI Zhong-liang;ZHANG Zhen-chao(Zhenjang Agricultural Research Institute in Jiangsu Hlly Region,Jurong,Jiangsu 212400,China;Shenyang Agricultural University,Shenyang 110866,China)
出处
《西南农业学报》
CSCD
北大核心
2024年第5期965-971,共7页
Southwest China Journal of Agricultural Sciences
基金
江苏省重点研发计划(现代农业)(BE2021376)。
