摘要
目的了解安徽省部分地区硬蜱蜱种种类、蜱种分布及蜱虫携带无形体情况,为蜱传无形体防控提供参考依据。方法于2023年4月至8月,在安徽省金寨县、含山县、旌德县、巢湖市等地通过布旗法和从动物身上采集硬蜱共计630只。用体式显微镜进行形态学鉴定,设计蜱特异性引物进行蜱种分子生物学鉴定。设计无形体16S rRNA特异性引物用巢式PCR检测单只蜱虫无形体携带情况。利用MEGA11.0构建系统发育树并分析。结果安徽省金寨县微小扇头蜱占18.8%(18/96)、长角血蜱占81.2%(78/96),含山县、巢湖市均为长角血蜱,旌德县均为微小扇头蜱。长角血蜱携带无形体阳性率为30.9%(102/330),包括牛无形体1.8%(6/330)、嗜吞噬细胞无形体21.8%(72/330)和一种uncultured无形体7.3%(24/330),微小扇头蜱携带嗜吞噬细胞无形体13.6%(18/132)。牛无形体和未培养无形体在游离蜱检测出,在24.4%的寄生蜱和15.8%的游离蜱检测出嗜吞噬细胞无形体。结论安徽省调查县的蜱种包括长角血蜱和微小扇头蜱,长角血蜱和微小扇头蜱及其游离蜱和寄生蜱均携带无形体。
The distribution of Ixodes and Anaplasma carried by Ixodes ticks in Anhui Province was clarified as reference for prevention and control of anaplasmosis.In total,630 hard ticks were collected from Jinzhai County,Hanshan County,Jingde County and Chaohu City in Anhui Province from April to August 2023.Ticks were identified by morphological analysis and 16S rRNA sequencing.Nested PCR with Anaplasma species-specific primers were used to detect 16S rRNA of Anaplasma species carried by ticks.A phylogenetic tree was constructed using MEGA11.0 software.Of the identified ticks,18.8%(18/96)were Rhipicephalus microplus and 81.2%(78/96)were Haemaphysalis longicorni in Jinzhai County of Anhui Province,all were H.longicorni in Hanshan County and Chaohu City,while the main species in Jingde County was R.microplus.The positive rate of Anaplasma carried by H.longicornis was 30.9%(102/330),which included A.bovis at 1.8%(6/330),A.phagocytophilum at 21.8%(72/330)and uncultured Anaplasma species at 7.3%(24/330).R.microplus was positively correlated to A.bovis(13.6%,18/132).The uncultured Anaplasma species was mainly detected in host-free ticks.A.phagocytophilum was detected in 24.4%of parasitic ticks and 15.8%of host-free ticks.The positive rates of host-free and parasitic ticks were 19.9%and 17.8%,respectively.These results show that H.longicornis and R.microplus were the dominant ticks in several counties of Anhui Province.H.longicornis and R.microplus as well as free and parasitic ticks all carried Anaplasma.
作者
赵雪
Lerato Khakhane
陆皖晴
吴鑫云
宋芳菲
新宇
张莹莹
邢晨
徐可瀚
何茂章
柳燕
ZHAO Xue;Lerato Khakhane;LU Wan-qing;WU Xin-yun;SONG Fang-fei;XIN Yu;ZHANG Ying-ying;XING Chen;XU Ke-han;HE Mao-zhang;LIU Yan(Department of Microbiology,Basic Medical College,Anhui Medical University,Hefei 230032,China)
出处
《中国人兽共患病学报》
CAS
CSCD
北大核心
2024年第4期302-307,共6页
Chinese Journal of Zoonoses
基金
安徽省重点研发长三角科技合作项目(No.2022i01020022)
安徽医科大学科研提升计划项目(No.2020xkjT003)联合资助。
关键词
蜱
蜱种鉴定
长角血蜱
微小扇头蜱
无形体
安徽省
tick
identification of tick species
Haemaphysalis longicorni
Rhipicephalus microplus
Anaplasma
Anhui Province
