摘要
染色体核型分析是细胞遗传学研究的基础,是研究物种演化、分类以及染色体结构与功能间关系不可或缺的重要手段。本实验采用秋水仙素溶液浸泡、KCl低渗、卡诺固定液固定、空气干燥和Giemsa染色等经典方法,对盐水浮游动物短角异剑水蚤(Apocyclops royi)的染色体数目和核型进行观察分析,同时比较了不同浓度的秋水仙素、KCl低渗时间以及蚤体解离方式的制片效果。结果表明,短角异剑水蚤染色体的数目为2n=12,全部为中部着丝点染色体,总臂数NF=24;在0.10%秋水仙素处理45~60 min和KCl低渗60~80 min条件下,以整体解离法制片的效果最好。研究结果可为短角异剑水蚤的细胞遗传学和盐水桡足类种质鉴定与保护提供科学参考。
Chromosome karyotype analysis serves as a fundamental tool in cell genetics research,facilitating in-vestigations into species evolution,classification,and the relationship between chromosome structure and func-tion.This study employed classic methods,including colchicine solution soaking,low-concentration KCL treat-ment,Carnoy’s fixative,air-drying,and Giemsa staining,to observe and analyze the chromosome number and karyotype of the saltwater copepod Apocyclops royi.Additionally,it compared the slide-making effects of vari-ous colchicine concentrations,low osmotic pressure duration,and copepod dissociation methods.The findings revealed that the chromosome number of A.royi was 2n=12,consisting entirely of metacentric chromosomes,totaling NF=24 arms.Optimal slide preparation was achieved using a complete dissociation method with 0.10%colchicine concentration,45-60 minutes of treatment,and 60-80 minutes of low-concentration KCL treatment.These research outcomes offer valuable insights for cell genetics research and germplasm identification and pro-tection of saltwater copepods.
作者
庞金池
万凯宏
谢玺
王庆志
魏杰
PANG Jinchi;WAN Kaihong;XIE Xi;WANG Qingzhi;WEI Jie(Liaoning Provincial Key Laboratory for Hydrobiology,College of Fisheries and Life Science,Dalian Ocean University,Dalian 116023,China;Dalian Key Laboratory of Genetic Resources for Marine Shellfish,Liaoning Ocean and Fisheries Science Research Institute,Dalian 116023,China)
出处
《渔业研究》
2024年第3期236-241,共6页
Journal of Fisheries Research
基金
辽宁省教育厅项目(LJKMZ20221102)。
