摘要
试验旨在获取具备木聚糖酶活性的发酵稻谷壳粉,以提高稻谷壳粉的饲用价值。试验用黑曲霉对稻谷壳粉进行固态发酵,以发酵产物中木聚糖酶活力为指标,采用单因素结合响应面法优化发酵条件并测量发酵产物营养物质的含量。结果显示,发酵基质质量为10 g、初始含水量为10.1 mL、添加1.0%的硫酸铵、培养温度为30.9℃、培养时间为3.1 d时,发酵产物中木聚糖酶活力最高,为135.643 U/mL,较优化前提高了3.6倍。在此条件下,发酵稻谷壳粉中粗蛋白和真蛋白含量均有提高,真蛋白的含量达12.35%,较发酵前提高了107.21%,粗纤维和粗脂肪含量有所降低,粗灰分含量提高。研究表明,发酵稻谷壳粉中木聚糖酶活力达到饲用标准,提高了蛋白质含量,可以作为辅料制备饲料。
The purpose of the experiment was to obtain fermented rice husk powder with xylanase activity,so as to improve the feeding value of rice husk powder.In this experiment,rice husk powder was fermented by Aspergillus Niger in solid state.The xylanase activity in fermented products was used as an index,and the fermentation conditions were optimized by single factor combined with response surface methodology,and the nutrient content of fermented products was measured.The results showed that when the substrate mass was 10 g,the initial water content was 10.1 mL,1.0%ammonium sulfate was added,the culture temperature was 30.9℃,and the culture time was 3.1 d,the highest xylanase activity was 135.643 U/mL,which was 3.6 times higher than that before optimization.Under these conditions,the content of crude protein and true protein in fermented rice husk powder increased,the content of true protein reached 12.35%,which increased by 107.21%compared with that before fermentation,the content of crude fiber and crude fat decreased,and the content of crude ash increased.The study indicates that the xylanase activity in fermented rice husk powder reached the feeding standard,and the protein content is increased,which can be used as an auxiliary material to prepare feed.
作者
冯庆玲
黄宝梅
谢君铨
林继辉
谢勇武
FENG Qing-ling;HUANG Bao-mei;XIE Jun-quan;LIN Ji-hui;XIE Yong-wu
出处
《饲料研究》
CAS
北大核心
2024年第9期93-98,共6页
Feed Research
基金
教育厅中青年科技项目(项目编号:JAT210618)
《生物工程》省级一流本科专业建设点项目(项目编号:SJZY-2020-02)。
关键词
黑曲霉
稻谷壳粉
木聚糖酶活力
饲料辅料
Aspergillus niger
rice husk powder
xylanase activity
feed auxiliary material
