摘要
目的:研究中药材大黄、五倍子、知母浸出液的体外抑菌效果。方法:用水提法对大黄、五倍子、知母进行提取,将各提取液浓缩成不同浓度后制成药敏片,通过不同作用时间、作用浓度以及最小抑菌浓度实验观察3味中药材对大肠杆菌和金黄色葡萄球菌的抑菌效果。结果:大黄提取液对大肠杆菌和金黄色葡萄球菌的抑菌程度属于中度敏感和高度/极度敏感;五倍子提取液对大肠杆菌和金黄色葡萄球菌的抑菌程度属于高度敏感和中度敏感;知母提取液对大肠杆菌和金黄色葡萄球菌的抑菌程度属于低/中度敏感。3味中药材的抑菌效果均存在时效性。五倍子、大黄、知母对大肠杆菌的最小抑菌浓度(MIC)分别为32 mg/mL、45 mg/mL、63 mg/mL;对金黄色葡萄球菌作用效果最好的是大黄,MIC为38 mg/mL。结论:大黄和五倍子提取液有较好的抑菌效果,知母的抑菌作用不太明显。
Objective:The bacteriostatic effect of extracts of Dahuang,Wubeizi and Zhimu in vitro is studied.Methods:Dahuang,Wubeizi and Zhimu are extracted by water extraction method,and the extracts are concentrated into different concentrations to prepare drug sensitive tablets.The antibacterial effects of three kinds of Chinese medicinal materials on Escherichia coli and Staphylococcus aureus are observed by experiments with different action time,action concentration and minimum inhibitory concentration.Results:Dahuang extract is moderately sensitive to Escherichia coli and highly sensitive to Staphylococcus aureus.The inhibitory degree of Wubeizi extract to Escherichia coli and Staphylococcus aureus is highly sensitive and moderately sensitive.The degree of Wubeizi extract to Escherichia coli and Staphylococcus aureus is low sensitivity and medium sensitivity.The antibacterial effect of the three kinds of Chinese medicinal materials is time-effective.The minimum inhibitory concentration(MIC)of Wubeizi,Dahuang and Zhimu against E.coli are 32 mg/mL,45 mg/mL and 63 mg/mL,respectively.Dahuang has the best effect on Staphylococcus aureus,with a MIC of 38 mg/mL.Conclusion:Dahuang and Wubeizi extract have good bacteriostatic effect,but the bacteriostatic effect of Zhimu is not obvious.
作者
江水芳
朱雨洁
宋纪池
康杰
曾叶芝
艾珏萍
JIANG Shuifang;ZHU Yujie;SONG Jichi;KANG Jie;ZENG Yezhi;AI Jueping(Changsha Medical University,Changsha 410219,China)
出处
《生物化工》
CAS
2024年第2期104-107,共4页
Biological Chemical Engineering
基金
湖南省教育厅资助项目(S202110823045,S202210823067)
湖南省教育厅科学研究项目一般项目(21C0866)
湖南省大学生创新训练项目(湘教通〔2022〕174号-4599)。
关键词
大黄
五倍子
知母
体外抑菌
Dahuang
Wubeizi
Zhimu
in vitro bacteriostasis
