摘要
Sensitive detection of Staphylococcus aureus enterotoxin B(SEB)is of importance for preventing food poisoning from threatening human health.In this work,an electrochemical and colorimetric dual-signal detection assay of SEB was developed.The probe(Ab2/AuPt@Fe-N-C)was bound to SEB captured by Ab1,where the Ab2/AuPt@Fe-N-C triggered methylene blue degradation and resulted in the decrease of electrochemical signal.Furthermore,the probe catalyzed the oxidation of 3,3’,5,5’-tetramethyl biphenyl to generate a colorimetric absorbance at 652 nm.Once the target was captured and formed a sandwich-like complex,the color changed from colorless to blue.SEB detection by colorimetric and electrochemical methods showed a linear relationship in the concentration ranges of 0.0002-10.0000 and 0.0005-10.0000 ng/mL,with limits of detection of 0.0667 and 0.1670 pg/mL,respectively.The dual-signal biosensor was successfully used to detect SEB in milk and water samples,which has great potential in toxin detection in food and the environment.
基金
This work was financially supported by Major Science and Technology Project of Yunnan Province(202302AE090022)
Key Research and Development Program of Yunnan(202203AC100010)
the National Natural Science Foundation of China(32160597,32160236,32371463)
National Key Research and Development Program of China(2022YFC2601604)
Cardiovascular Ultrasound Innovation Team of Yunnan Province(202305AS350021)
Spring City Plan:the High-level Talent Promotion and Training Project of Kunming(2022SCP001)
the second phase of“Double-First Class”Program Construction of Yunnan University.
