摘要
为筛选出高产β-石竹烯(BCP)菌株,该研究通过顶空固相微萃取(HS-SPME)-气相色谱质谱(GC-MS)法对清香型白酒和大曲中香气成分进行检测,采用传统分离技术对大曲中产β-石竹烯(BCP)菌株进行初筛,将初筛菌株接种麸曲测定BCP含量进行复筛,并采用分子生物学技术对其进行菌种鉴定。对麸曲中β-石竹烯提取条件进行优化,并将筛选菌株进行小坛发酵应用。结果表明,该清香型原酒中β-石竹烯含量为0.02 g/L,大曲中β-石竹烯相对含量为0.85%。从大曲中初筛出5株霉菌(SDA1~SDA5),通过复筛得到β-石竹烯相对含量最高(1.04%)菌株SDA3被鉴定为戴尔根霉(Rhizopus delemar)。在提取试剂为无水乙醇、提取时间为2 h条件下,BCP含量为37.98 mg/L。与仅添加大曲相比,加入菌株SDA3强化麸曲用于小坛发酵,其产白酒中β-石竹烯的含量从13.65 mg/L提高至24.65 mg/L。
In order to screen the high-yieldβ-caryophyllene(BCP)strains,the aroma components in light-flavor(Qingxiangxing)Baijiu and Daqu was detected by headspace solid-phase microextraction(HS-SPME)-GC-MS.Theβ-caryophyllene-producing strains were preliminarily screened by traditional isolation techniques,Fuqu was inoculated with primary screening strains to re-screening byβ-caryophyllene content determination,and identified by molecular biology technique.The extraction conditions ofβ-caryophyllene in Fuqu were optimized,and the screened strains were applied in small tank fermentation.The results showed that theβ-caryophyllene content in the light-flavor original liquor was 0.02 g/L,and the relative content ofβ-caryophyllene in Daqu was 0.85%.Five strains of mold(SDA1-SDA5)were preliminarily screened,and the strain SDA3 with the highest relative content ofβ-caryophyllene(1.04%)was identified as Rhizopus delemar.Theβ-caryophyllene content was 37.98 mg/L when the extraction reagent was anhydrous ethanol and the extraction time was 2 h.Compared with the addition of Daqu alone,theβ-caryophyllene content in liquor produced by adding strain SDA3 to enhance Fuqu for small tank fermentation was increased from 13.65 mg/L to 24.65 mg/L.
作者
牟飞燕
何夷敏
夏博宇
董孝元
陈晖
常煦
陈茂彬
方尚玲
MOU Feiyan;HE Yimin;XIA Boyu;DONG Xiaoyuan;CHEN Hui;CHANG Xu;CHEN Maobin;FANG Shangling(Cooperative Innovation Center of Industrial Fermentation(Ministry of Education&Hubei Province),School of Food and Biological Engineering,Hubei University of Technology,Wuhan 430068,China;Huanghelou Distillery Co.,Ltd.,Wuhan 430000,China;Angel Yeast Co.,Ltd.,Yichang 443000,China)
出处
《中国酿造》
CAS
北大核心
2024年第4期91-97,共7页
China Brewing
基金
“十三五”国家重点研发计划重点专项(2016YFD0400500)
湖北工业大学研究生教学改革研究项目(校2021057)。
关键词
β-石竹烯
大曲
白酒
霉菌
筛选
鉴定
强化麸曲
β-caryophyllene
Daqu
Baijiu
mold
screening
identification
fortified Fuqu
