摘要
本工作合成了三层核壳结构的上转换发光纳米粒子,其中第一层是惰性核,第二层为发光层,为了增强上转换发光强度,又在表面包覆了第三层惰性壳层。该材料粒径均一、分散性良好,其发光层厚度约为2.4nm,惰性壳层厚度约为2.9nm。在其表面修饰了细胞色素C的适配体链及互补链,在适配体的3’端修饰了BHQ3基团,能够猝灭上转换纳米粒子在655nm波长处的发光。当细胞色素C存在时,适配体与细胞色素C结合从而离开其表面,使655nm处的发光恢复。在检测过程中,540nm处的发光强度不会发生变化,可以用作细胞色素C的比率荧光检测。结果表明,当细胞色素C浓度在5~80μmol/L范围时,发光信号恢复程度与细胞色素C浓度呈线性相关,相关系数为0.998,检出限为1μmol/L。所建立方法可为细胞色素C的荧光检测提供一种新的技术思路。
In this work,core-shell-shell upconversion luminescence nanoparticles(UCNPs)are synthesized,with an inert core,a confined emitter layer,and an inert shell on the emitter layer enhancing the upconversion luminescence intensity.The as-prepared upconversion nanoparticles possess uniform particle size and good dispersion,and the thickness of the emitter layer and the inert shell are about 2.4 and 2.9 nm,respectively.By modification of the surface of the nanoparticles with the aptamer cytochrome C and its complementary chain,with BHQ3 labelled at the 3’end of the aptamer,the nanoparticles could be applied to quench the luminescence of the materials at 655 nm.In the presence of cytochrome C,the aptamer binds to cytochrome C and detaches from the surface of upconversion nanoparticles,resulting in recovery of emission at 655 nm.In this assay,the emission intensity at 540 nm does not change and can be used as a ratiometric luminescence detection of cytochrome C.The results show that the recovery of luminescence has a good linear relationship with the concentration of cytochrome C in the range of 5 to 80μmol/L(R 2=0.998),and the detection limit is 1μmol/L.The method provides a new protocol for the luminescence detection of cytochrome C.
作者
董佳瑶
易静
刘浏
于贺
唐宏武
DONG Jiayao;YI Jing;LIU Liu;YU He;TANG Hongwu(College of Chemistry and Molecular Sciences,Wuhan University,Wuhan 430072)
出处
《分析科学学报》
CAS
CSCD
北大核心
2024年第2期125-131,共7页
Journal of Analytical Science
基金
国家自然科学基金(22277096,21827808)。
