摘要
目的建立高效液相色谱法同时测定香砂和胃丸中芸香柚皮苷、柚皮苷、橙皮苷、新橙皮苷、和厚朴酚以及厚朴酚的含量。方法HPLC法色谱柱采用依利特SinoChrom ODS-BP C 18柱(250 mm×4.6 mm,5μm);以0.2%磷酸水溶液(A)-乙腈(B)为流动相,梯度洗脱;流速1.0 mL/min;检测波长230 nm;进样量10μL;柱温30℃。结果芸香柚皮苷、柚皮苷、橙皮苷、新橙皮苷、和厚朴酚、厚朴酚分别在3.274~32.740μg/mL(r=0.9998)、6.882~68.820μg/mL(r=0.9999)、20.966~209.660μg/mL(r=0.9999)、5.754~57.540μg/mL(r=0.9999)、1.980~19.800μg/mL(r=0.9999)、2.274~22.740μg/mL(r=0.9999)范围内线性关系良好;平均加样回收率分别为101.00%(RSD=0.71%)、101.92%(RSD=0.60%)、102.37%(RSD=0.58%)、98.05%(RSD=0.43%)、100.98%(RSD=0.81%)、100.64%(RSD=0.83%)。结论建立的HPLC法灵敏度高、操作简便、专属性好,可为香砂和胃丸的质量控制提供依据。
Objective To establish an high-performance liquid chromatography(HPLC)method for the simultaneous determination of narirutin,naringin,hesperidin,neohesperidin,honokiol and magnolol in Xiangsha Hewei pills.Methods The separation was performed on an Elite Sino Chrom ODS-BP C 18 column(250 mm×4.6 mm,5μm)with 0.2%phosphoric acid aqueous solution(A)-acetonitrile(B)as mobile phase for gradient elution.The flow rate was 1.0 mL/min,the detection wavelength was 230 nm,the injection volume was 10μL,and the column temperature was 30℃.Results Narirutin,naringin,hesperidin,neohesperidin,honokiol and magnolol showed good linear relationships within the ranges of 3.274-32.740μg/mL(r=0.9998),6.882-68.820μg/mL(r=0.9999),20.966-209.660μg/mL(r=0.9999),5.754-57.540μg/mL(r=0.9999),1.980-19.800μg/mL(r=0.9999),2.274-22.740μg/mL(r=0.9999),respectively.The average recoveries were 101.00%(RSD=0.71%),101.92%(RSD=0.60%),102.37%(RSD=0.58%),98.05%(RSD=0.43%),100.98%(RSD=0.81%),100.64%(RSD=0.83%),respectively.Conclusion The established HPLC method has the advantages of high sensitivity,simple operation and good specificity,which can provide a basis for the quality control of Xiangsha Hewei pills.
作者
马莎
崔庆德
王黛莹
吕晋
李桂香
MA Sha;CUI Qingde;WANG Daiying;L Jin;LI Guixiang(Department of Pharmaceutical Preparations,School of Pharmacy,Qujing Medical College,Qujing 655000,China;Chemical Room,Qujing Food and Drug Inspection and Testing Center;Department of Pharmacy,Qujing Maternal and Child Health Hospital;Department of Drug Analysis,School of Pharmacy,Qujing Medical College)
出处
《山西医科大学学报》
CAS
2024年第4期509-514,共6页
Journal of Shanxi Medical University
基金
曲靖医学高等专科学校开放课题项目(2023YXYKF004)。
