摘要
为比较不同品种葡萄多糖含量以及体外抗氧化活性,以克伦生葡萄、云南玫瑰香小葡萄和阳光玫瑰葡萄3种鲜品葡萄为研究对象,以提取率和多糖含量为评价指标,利用热水提取法、Sevag脱蛋白得到3种葡萄多糖,进行红外光谱分析,采用体外测试法考察不同品种葡萄多糖的抗氧化活性.结果表明:3种葡萄多糖提取率差异不显著,云南玫瑰香小葡萄的多糖含量最高为5.74%,意味着云南玫瑰香小葡萄在多糖含量存在优势.红外光谱图说明三种样品都具有相似多糖特征峰.不同品种葡萄多糖有不同的抗氧化活性,其中阳光玫瑰葡萄多糖在清除DPPH自由基方面能达到82.38%,云南玫瑰香小葡萄多糖在清除羟基自由基方面能达到83.32%,为进一步开发利用葡萄多糖提供了研究依据.
In order to compare the content and antioxidant activities in vitro of different kinds grape polysaccharides,three fresh kinds of Crison grape,Yunnan Muscat Hamburg Xiao grape and Shine Muscat grape were studied.With extraction rate and polysaccharide content as evaluation index,three grape polysaccharides were obtained by hot water extraction and Sevag deproteinization,respectively,the different varieties of grape polysaccharides were investigated by infrared spectroscopy.The antioxidant activities of polysaccharides from different varieties were investigated by in vitro.The results showed that there was no significant difference in polysaccharides extraction yield among the three kinds of grapes,and the highest polysaccharides content of Yunnan Muscat Hamburg Xiao grape was 5.74%,which meant that Yunnan Muscat Hamburg Xiao grape had advantages in polysaccharide content.The infrared spectra showed that the three kinds of grapes had similar polysaccharides characteristic peaks.Different varieties of grape polysaccharides had different antioxidant activities,among which Shine Muscat grape polysaccharides was as high as 82.38%in scavenging DPPH free radical,and Yunnan Muscat Hamburg Xiao grape polysaccharides was up to 83.32%in scavenging hydroxyl free radical,which provided the research basis for further development and utilization of grape polysaccharides.
作者
曾小彤
林劲松
陈立强
张常洪
罗莎莎
魏伟
ZENG Xiaotong;LIN Jinsong;CHEN Liqiang;ZHANG Changhong;WEI Wei(Chemistry and chemical engineering school of Sichuan University of Arts and Science;Key Laboratory of Low-cost Rural Environmental Treatment Technology of Sichuan Universities,Dazhou Sichuan 635000,China)
出处
《四川文理学院学报》
2024年第2期38-43,共6页
Sichuan University of Arts and Science Journal
基金
乡村低成本环境治理技术四川省高校重点实验室2022年度开放基金项目(XCH2022ZB-07)
2023年四川省大学生创新创业训练计划项目(S202310644061)。
关键词
葡萄
多糖
含量
抗氧化活性
Grape
polysaccharides
content
antioxidant activities
