摘要
为解析宁夏枸杞响应NaCl胁迫的ABA代谢分子调控机制,检测NaCl胁迫下宁夏枸杞叶片脱落酸(ABA)含量变化,并利用RNA-seq和qRT-PCR技术研究NaCl胁迫下ABA代谢相关基因的差异表达规律。结果表明,不同浓度NaCl胁迫下,宁夏枸杞叶片中ABA含量随NaCl浓度的增加呈现增加趋势;通过转录组测序筛选得到21个ABA代谢相关的差异表达基因,从100 mmol/L、200 mmol/L、300 mmol/L NaCl处理下的宁夏枸杞叶片中检测到ABA代谢相关的差异表达基因分别有17、11、9个,qRT-PCR检测结果与转录组测序结果基本一致。宁夏枸杞通过相关基因的差异表达调控ABA代谢和信号通路,从而参与调控其响应NaCl胁迫。
To analyze the molecular regulation mechanism of ABA metabolism of Lycium barbarum in response to NaCl-induced stress,we detected ABA contents of L.barbarum leaves under NaCl stress,and used RNA-seq and qRT-PCR techniques to analyze the differential expression of ABA metabolism related genes.The results showed that ABA content in leaves of L.barbarum increased by NaCl stress.21 ABA metabolism-related genes were differentially expressed in L.barbarum,and 17,11 and 9 ABA metabolism-related genes were differentially expressed in 100 mmol/L,200 mmol/L and 300 mmol/L NaCl,respectively.Meanwhile,the results of qRT-PCR were consistent with the results of transcriptome sequencing.L.barbarum responded to NaCl stress through regulating ABA metabolism and signal pathway by differential expression of related genes.
作者
梁旺利
于雯静
胡进红
宋繁
王玲霞
梁文裕
LIANG Wangli;YU Wenjing;HU Jinhong;SONG Fan;WANG Lingxia;LIANG Wenyu(School of Life Sciences,Ningxia University,Yinchuan 750021,China)
出处
《西北农业学报》
CAS
CSCD
北大核心
2024年第4期664-672,共9页
Acta Agriculturae Boreali-occidentalis Sinica
基金
宁夏自然科学基金(2022AAC03083)
2022年度宁夏留学回国人员创新创业项目(宁人社函[2023]5号-5)
宁夏回族自治区科技创新团队项目(NXKJT2019002)~~
关键词
宁夏枸杞
NACL胁迫
ABA
代谢
差异基因表达
Lycium barbarum
NaCl stress
ABA
Metabolism
Differential expression of genes
