摘要
目的分析长链非编码RNA LINC01600在结直肠癌组织中的表达以及在临床结直肠癌诊断过程中的价值。方法选取2020年1月至2023年12月新乡医学院第一附属医院收治的37例结直肠癌患者的临床标本作为研究标本, 选择对应的癌旁组织作为对照样本。提取癌旁组织和结直肠癌组织的总RNA, 采用转录组学技术分析癌组织和癌旁组织差异表达的长链非编码RNA。选取差异最为显著的长链非编码RNA LINC01600作为研究对象。采用荧光定量聚合酶链反应技术检测结直肠癌组织和癌旁组织中LINC01600的表达水平。制作受试者工作特征曲线分析LINC01600对结直肠癌的临床诊断效能。采用单因素方差分析不同临床分期, 不同分化程度患者LINC01600表达水平。并分析LINC01600和患者预后的关系。结果结直肠癌和癌旁组织共检出39 123个长链非编码RNA, 其中包括2 018个新的长链非编码RNA。差异分析显示, 其中有1 626个差异表达的长链非编码RNA, 其中1 019个上调基因, 607个下调基因。长链非编码RNA主要富集于核糖体通路、柠檬酸循环通路和RNA剪接相关通路。本研究选取差异最为显著的上调长链非编码RNA LINC01600进行研究。癌旁组织长链非编码RNA LINC01600表达水平(0.85±0.10)明显低于结直肠癌组织(1.99±0.31), 差异有统计学意义(t=21.390, P<0.05), 中高分化患者肿瘤组织中长链非编码RNA LINC01600表达水平(1.70±0.11)显著低于低分化患者(2.19±0.24), 差异有统计学意义(t=7.510, P<0.05)。淋巴结转移患者结直肠癌组织长链非编码RNA LINC01600表达水平(2.24±0.23)显著高于结直肠癌组织(1.80±0.20), 差异有统计学意义(t=6.229, P<0.05)。Ⅲ期患者结直肠癌组织长链非编码RNA LINC01600表达水平(2.19±0.32)显著高于结直肠癌组织(1.92±0.27), 差异有统计学意义(t=2.796, P<0.05)。LINC01600高表达组结直肠癌患者中位生存时间(14个月)明显低于LINC01600低表达患者(26个月), 差异有统计学意义(Log-rank=4.618, P<0.05)。肿瘤组织长链非编码RNA LINC01600诊断结直肠癌的曲线下面积(AUC)为0.814[95%可信区间(CI)=0.754~0.968, P<0.05]。LINC01600敏感性为92.45%, 特异性为89.36%。结论长链非编码RNA LINC01600在结直肠癌患者肿瘤组织中达水平明显高于癌旁组织, 有助于临床结直肠癌患者术前和预后诊断。
Objective To analyze the expression of long-chain non coding RNA LINC01600 in colorectal cancer(CRC)tissue and its value in the clinical diagnosis of CRC.Methods Clinical specimens of 37 patients with CRC admitted to our hospital from January 2020 to December 2023 were selected as research objects,and corresponding adjacent cancerous tissues were selected as controls.The total RNA was extracted from adjacent cancerous tissues and CRC tissues,and transcriptomic techniques were used to analyze the differentially expressed long chain non coding RNA between cancer tissues and adjacent cancerous tissues.The long-chain non coding RNA LINC01600 was selected as the research object.The expression level of LINC01600 in CRC cancer tissues and adjacent tissues was detected by fluorescence quantitative polymerase chain reaction.A subject work characteristic curve was drawn to analyze the clinical diagnostic efficacy of LINC01600 for CRC.The expression levels of LINC01600 in patients with different clinical stages and degrees of differentiation were analyzed by one-way ANOVA.The relationship between LINC01600 and patient prognosis was analyzed.Results A total of 39123 long chain non coding RNAs were detected in CRC and adjacent tissues,including 2018 new long chain non coding RNAs.Differential analysis showed that there were 1626 differentially expressed long chain non coding RNAs,including 1019 upregulated genes and 607 downregulated genes.Long chain non coding RNAs were mainly enriched in ribosomal pathways,citrate cycle pathways,and RNA splicing related pathways.The expression level of long chain non coding RNA LINC01600 in adjacent cancer tissues(0.85±0.10)was significantly lower than that in CRC tissues(1.99±0.31,t=21.390,P<0.05).The expression level of long chain non coding RNA LINC01600 in tumor tissues of medium to high differentiation patients(1.70±0.11)was significantly lower than that of low differentiation patients(2.19±0.24,t=7.510,P<0.05).The expression level of long chain non coding RNA LINC01600 in CRC tissues of patients with lymph node metastasis(2.24±0.23)was significantly higher than that in CRC tissues(1.80±0.20,t=6.229,P<0.05).The expression level of long-chain non coding RNA LINC01600 in stageⅢCRC tissues(2.19±0.32)was significantly higher than that in CRC tissues(1.92±0.27,t=2.796,P<0.05).The median survival time(14 months)of CRC patients with high expression of LINC01600 was significantly lower than that in patients with low expression of LINC01600(26 months)(Log-rank=4.618,P<0.05).The area under curve(AUC)of long chain non coding RNA LINC01600 in tumor tissue for diagnosing CRC was 0.814[95%confidence interval(CI)=0.754-0.968,P<0.05].LINC01600 had a sensitivity of 92.45%and a specificity of 89.36%.Conclusion The level of long-chain non coding RNA LINC01600 in tumor tissue of CRC patients is significantly higher than that in adjacent tissues,and can be used as an auxiliary marker for preoperative and prognostic diagnosis in CRC.
作者
李秀庚
王高翔
蒲亚陆
Li Xiugeng;Wang Gaoxiang;Pu Yalu(Department of Colorectal and Anorectal Surgery,the First Affiliated Hospital of Xinxiang Medical College,Weihui 453100,China;Department of Life Science Research Center,the First Affiliated Hospital of Xinxiang Medical College,Weihui 453100,China)
出处
《中华实验外科杂志》
CAS
2024年第2期344-347,共4页
Chinese Journal of Experimental Surgery
关键词
长链非编码RNA
结直肠癌
Long chain non coding RNA
Colorectal cancer
