摘要
目的研究淋巴样增强结合因子1(lymphoid enhancer-binding factor 1,LEF1)在B细胞慢性淋巴增殖性疾病(B cell chronic lymphoproliferative disorders,B-CLPD)中的表达情况,探讨在B-CLPD亚型诊断中的价值。方法回顾性分析58例B-CLPD患者骨髓或淋巴结标本和20例对照组标本,采用免疫组化的方法,检测LEF1的表达情况。结果慢性淋巴细胞白血病(chronic lymphocytic leukemia,CLL)20例,LEF1阳性16例,阳性率为80%;套细胞淋巴瘤阳性为1/12;滤泡性淋巴瘤为1/5;边缘区淋巴瘤为0/11;淋巴浆细胞淋巴瘤为0/8;毛细胞白血病0/2;20例非恶性血液病患者未见LEF1表达;LEF1表达在CLL组差异有统计学意义(P=0.000);LEF1的表达和CD200、CLL积分等CLL的相关指标具有相关性(P<0.05)。4例LEF1阴性的CLL患者中,2例+12染色体异常,检出率高于LEF1阳性组(P>0.05)。结论LEF1在慢性淋巴细胞白血病患者的诊断和鉴别诊断中,有较好的灵敏性和特异性,是B-CLPD鉴别诊断的良好指标。
Objective To investigate the expression of lymphoid enhancer binding factor 1(LEF1)in B cell chronic lymphoproliferative disorders(B⁃CLPD)and estimate its value in the differential diagnosis of the subtype of B⁃CLPD.Methods A retrospective study was conducted on 58 patients diagnosed with B⁃CLPD by using bone marrow biopsy samples or lymphnode biopsy samples from Hematology Department of The Second People's Hospital of Wuhu from September 2018 to June 2023,as well as 20 bone marrow biopsy samples which were diagnosis as non⁃hematologic malignancy in the control group.Immunohistochemical method was used to detect the expression of LEF1 in 78 samples,and statistical analysis was conducted.Results In all 78 cases,16 of 20 chronic lymphocytic leukemia(CLL)patients were LEF1 positive,the positive rate was 80%;mantle cell lymphoma 1/12;Follicular lymphoma 1/5;marginal zone cell lymphoma 0/11;Lymphoplasmacyticlymphom 0/8;hairy cell leukemia 0/2;in Control group no patient was LEF1 positive(P=0.000).The expression of LEF1 is correlated with CD200 and CLL score(P<0.05).In the LEF1 negative group with 4 CLL patients,2 were detected with+12 chromosomal abnormality,the detective rate was higher than that of the LEF1 positive group(P>0.05).Conclusion LEF1 was a sensitive and specific diagnosis marker in CLL and B⁃CLPD subtype.
作者
赵敏
倪平
翟惠莹
靳小可
杨玉琼
ZHAO Min;NI Ping;ZHAI Huiying;JIN Xiaoke;YANG Yuqiong(Department of Hematology,the Second People′s Hospital of Wuhu,Wuhu 241000,China;不详)
出处
《实用医学杂志》
CAS
北大核心
2024年第7期984-988,共5页
The Journal of Practical Medicine
基金
安徽省教育厅科学研究项目-重点项目(编号:2022AH051246)。
