摘要
目的 研究贵州苗族红酸汤对高脂饮食诱导型大鼠非酒精性脂肪肝(NAFLD)的保护作用及其可能机制。方法 根据国标法检测红酸汤抗氧化成分番茄红素、辣椒碱、乳酸及乙酸的含量;无特定病原体(SPF)级雄性SD大鼠30只,按照体质量随机分为酸汤组、模型组和正常组(每组10只),酸汤组大鼠喂养高脂饲料并根据体质量按照8 g/kg灌胃红酸汤,模型组大鼠喂养高脂饲料并灌胃等容积蒸馏水,正常组大鼠喂养普通饲料并灌胃等容积蒸馏水,每日记录大鼠体质量、摄食量;连续灌胃12周后,称量大鼠体质量并计算肥胖指数(Lee’s指数)和肝脏指数,测定血脂四项、谷丙转氨酶(ALT)及谷草转氨酶(AST)水平,血清总抗氧化能力(T-AOC)、丙二醛(MDA)、肿瘤坏死因子-α(TNF-α)及白细胞介素6(IL-6)含量;取各组大鼠肝脏苏木素-伊红染色(HE染色)和油红O染色,显微镜观察脂质沉积及肝组织学特征。结果 红酸汤抗氧化成分番茄红素、辣椒碱和有机酸的含量较丰富;动物实验结果表明,与模型组相比,酸汤组大鼠的体质量减轻,Lee’s指数和肝脏指数降低(P<0.05),血清甘油三酯(TG)、总胆固醇(TC)和低密度脂蛋白胆固醇(LDL-C)含量降低(P<0.05),高密度脂蛋白胆固醇(HDL-C)含量升高(P<0.05),血清ALT和AST水平下降(P<0.05),血清T-AOC、MDA、TNF-α和IL-6含量降低(P<0.05);HE染色和油红O染色结果显示红酸汤干预能预防肝脏组织脂肪变性和肝细胞脂质沉积。结论 贵州苗族红酸汤对NAFLD有一定预防作用,可能与其调节血脂紊乱、改善肝脏脂肪变性和脂质沉积、减轻机体氧化应激和慢性炎症有关。
Objective To detect the protective effect and possible mechanism of Guizhou Miao red sour soup on hepatic steatosis and lipid deposition in rats with nonalcoholic fatty liver disease(NAFLD).Methods The content of antioxidants in Guizhou Miao red sour soup was detected according to the National Standard Test.Rats in the Normal group were fed with ordinary foodstuffs and equal volume distilled water,rats in the Model group were fed with high fat foodstuffs and equal volume distilled water,and rats in the red sour soup group were fed with high fat foodstuffs and gavage with 8 g/kg dose of red sour soup.Rat food intake and body weight were recorded daily.After constant gavage for 12 weeks,the body weight of rats was weighed,and the levels of Lee's index,liver index,serum lipid,alanine aminotransferase(ALT),and aspartate aminotransferase(AST)were measured,the levels of total antioxidant capability(T-AOC),malondialdehyde(MDA),tumor necrosis factor-α(TNF-α),and interleukin 6(IL-6)were surveyed,and the liver was stained with H&E and oil red O.Results The antioxidant contents such as lycopene,capsaicin and organic acids in Miao red sour soup were rather rich.Compared with the model group,Miao red sour soup group lost weight,Lee's index and Liver index decreased,and the contents of serum triglyceride(TG),total cholesterol(TC)and low density lipoprotein cholesterol(LDL-C)decreased(P<0.05),while the content of high density lipoprotein cholesterol(HDL-C)increased(P<0.05),and the levels of serum ALT and AST decreased(P<0.05).Serum AOC,MDA,TNF-α,and IL-6 decreased(P<0.05),reducing oxidative stress and chronic inflammation.H&E staining and oil red O staining showed that the hepatic steatosis and lipid deposition were prevented.Conclusion Guizhou Miao red sour soup has a certain preventive and therapeutic effect on NAFLD,which may be related to regulating blood lipid disorder,improving liver steatosis and lipid deposition,reducing oxidative stress and chronic inflammation.
作者
王乃经
陈云云
陈莎
唐扩
王惠群
WANG Naijing;CHEN Yunyun;CHEN Sha;TANG Kuo;WANG Huiqun(School of Public Health,the Key Laboratory of Environmental Pollution Monitoring and Disease Control,Ministry of Education,Guizhou Medical University,Guiyang 561113,Guizhou,China)
出处
《贵州医科大学学报》
CAS
2024年第2期228-234,共7页
Journal of Guizhou Medical University
基金
贵州省2022年大学生创新创业训练计划项目(S202210660120)。
关键词
红酸汤
非酒精性脂肪肝
脂质沉积
氧化应激
慢性炎症
red sour soup
nonalcoholic fatty liver disease
lipid deposition
oxidative stress
chronic inflammation
