摘要
采用UASB反应器,接种厌氧氨氧化菌自富集启动的PDA污泥,在温度由25℃下降到19℃条件下逐步将进水氮浓度从20mg/L提高到100mg/L(即氮负荷从3.2g/(m^(3)·d)提高到4.0g/(m^(3)·d)),反应器共运行115d,总氮去除率最高可达到89.9%.16S rRNA基因测序结果表明,Candidatus Brocadia作为主要厌氧氨氧化功能菌在生物填料中丰度达到3.7%,短程反硝化功能菌Thaurea在絮体污泥中的丰度最高达15.7%.宏基因组学分析显示与短程反硝化相关的基因Nar,Nap和与厌氧氨氧化相关的Nir,Hzs和Hdh基因表达程度较高.
A UASB inoculated with PDA sludge initiated by anammox bacteria self-enrichment was used to gradually increase the influent nitrogen concentration from 20mg/L to 100mg/L at 25℃down to 19℃(the nitrogen load increased from 3.2g/(m^(3)·d)to 4.0g/(m^(3)·d)).The reactor was operated for 115 days with a maximum total nitrogen removal efficiency of 89.9%.16S rRNA gene sequencing results showed that Candidatus Brocadia as the main anammox functional bacterium reached 3.7%abundance in the bio-carriers and the partial denitrifying functional bacterium Thaurea reached a maximum abundance of 15.7%in the floc sludge.Metagenomic analysis showed that the genes Nar and Nap,associated with partial denitrification,and Nir,Hzs and Hdh,associated with anammox,were fully expressed.
作者
马雨晴
王博
李笑迪
MA Yu-qing;WANG Bo;LI Xiao-di(Engineering Research Center of Beijing,National Engineering Laboratory for Advanced Municipal Wastewater Treatment and Reuse Technology,Beijing University of Technology,Beijing 100124,China)
出处
《中国环境科学》
EI
CAS
CSCD
北大核心
2024年第3期1307-1313,共7页
China Environmental Science
基金
国家自然科学基金资助项目(22176007)
(校拨)研究生培养相关经费-环境与生命学部-培育基金项目(PY202305)。
