摘要
目的:建立火锅底料中喹诺酮类化合物的测定方法。方法:样品经0.1 mol·L^(-1) EDTA-Mcllvaine溶液提取,5%甲醇水溶液净化后上液相色谱-串联质谱仪分析。采用SHIMADZU C_(18)色谱柱(2.1 mm×100 mm,3μm)进行分离,流动相为0.1%甲酸-乙腈,梯度洗脱。采用电喷雾离子源,正离子扫描。结果:该方法线性关系良好,相关系数≥0.99;样品在1.0~20.0μg·kg^(-1)的添加水平下加标回收率为65.20%~110.20%。结论:该方法适用于火锅底料中喹诺酮类化合物的测定,简单快速,灵敏度高,重复性好。
Objective:To establish a method for the determination of quinolones in hot pot base.Method:Hot pot base sample were extracted by 0.1 mol·L^(-1) EDTA-Mcllvaine solution,purified by 5%methanol aqueous solution,and analyzed by liquid chromatography-tandem mass spectrometry.Separation was performed using a column SHIMADZU C_(18) column(2.1 mm×100 mm,3μm)with 0.1%formic acid-acetonitrile as a mobile phase and gradient elution.The ion source is an electrospray ion source,and the scanning method is positive ion scanning.Result:The method has a good linear relationship,and the correlation coefficient is greater than or equal to 0.99.The recoveries of spiked samples at spiked levels of 1.0~20.0μg·kg-1 ranged from 65.20%to 110.20%.Conclusion:This method is suitable for the determination of quinolones in hotpot seasonings,which is simple,rapid,sensitive and reproducible.
作者
康宗华
江露
KANG Zonghua;JIANG Lu(Hunan Kouweiwang Group Co.,Ltd.,Yiyang 413000,China;Hunan Tuopu Inspection Institute,Yiyang 413000,China)
出处
《食品安全导刊》
2024年第3期53-55,共3页
China Food Safety Magazine
关键词
液相色谱-串联质谱法
火锅底料
喹诺酮
liquid chromatography-tandem mass spectrometry
hot pot base
quinolones
