摘要
目的:探讨喉鳞状细胞癌(LSCC)组织miR-1207-5p、miR-186-5p表达水平与磷脂酰肌醇3-激酶/蛋白激酶B(PI3K/Akt)信号通路、临床病理特征和预后的关系。方法:选取2017年1月至2020年1月南充市中心医院收治的120例LSCC患者,取手术切除的LSCC组织和癌旁组织。检测miR-1207-5p、miR-186-5p、PI3K mRNA、Akt mRNA表达。患者出院后随访3年,统计总生存(OS)和无复发生存(RFS)情况。分析miR-1207-5p、miR-186-5p与PI3K、Akt的相关性以及影响LSCC患者预后的因素。结果:LSCC组织miR-1207-5p、miR-186-5p表达低于癌旁组织(P<0.05),PI3K mRNA、Akt mRNA表达高于癌旁组织(P<0.05)。LSCC组织miR-1207-5p、miR-186-5p表达与PI3K mRNA、Akt mRNA表达呈负相关(P<0.05)。肿瘤直径≥1 cm、低分化、TNM分期Ⅲ期、颈部淋巴结转移LSCC组织中miR-1207-5p、miR-186-5p表达低于肿瘤直径<1 cm、中高分化、TNM分期Ⅰ~Ⅱ期、无颈部淋巴结转移(P<0.05)。miR-1207-5p低表达、miR-186-5p低表达LSCC患者3年总生存(OS)率和无复发生存(RFS)率低于miR-1207-5p高表达、miR-186-5p高表达LSCC患者(P<0.05)。多因素COX回归分析显示TNMⅢ期、颈部淋巴结转移是LSCC患者复发和死亡的危险因素(P<0.05),高miR-1207-5p、高miR-186-5p是保护因素(P<0.05)。结论:LSCC组织中miR-1207-5p和miR-186-5p表达均下调,与LSCC恶性病理特征、PI3K/Akt信号通路激活以及低生存率有关。
Objective:To investigate the relationship between the expression levels of miR-1207-5p and miR-186-5p in laryngeal squamous cell carcinoma(LSCC)tissue and the phosphatidylinositol 3-kinase/protein kinase B(PI3K/Akt)signaling pathway,clinicopathological features and prognosis.Methods:120 patients with LSCC admitted to Nanchong Central Hospital from January 2017 to January 2020 were selected,and the surgically resected LSCC tissue and paracancer tissue were taken.The expression of miR-1207-5p,miR-186-5p,PI3K mRNA and Akt mRNA were detected.After discharge,the patient was followed up for 3 years to calculate the overall survival(OS)and recurrence free survival(RFS).The correlation of miR-1207-5p and miR-186-5p with PI3K and Akt and the factors affecting the prognosis of LSCC patients were analyzed.Results:The expressions of miR-1207-5p and miR-186-5p in LSCC tissue were lower than those in paracancer tissue(P<0.05),and the expressions of PI3K mRNA and Akt mRNA in LSCC tissue were higher than those in paracancer tissue(P<0.05).The expressions of miR-1207-5p and miR-186-5p in LSCC tissue were negatively correlated with the expressions of PI3K mRNA and Akt mRNA(P<0.05).The expressions of miR-1207-5p and miR-186-5p in LSCC tissue with tumor diameter≥1 cm,poorly differentiated,TNM stageⅢ,and cervical lymph node metastasis were lower than those in LSCC tissue with tumor diameter<1 cm,medium and highly differentiated,TNM stageⅠtoⅡ,and no cervical lymph node metastasis(P<0.05).The 3-year overall survival(OS)rate and relapse-free survival(RFS)rate of LSCC patients with low expression of miR-1207-5p and low expression of miR-186-5p were lower than those with high expression of miR-1207-5p and high expression of miR-186-5p LSCC patients(P<0.05).Multivariate COX regression analysis showed that TNM stageⅢand cervical lymph node metastasis were risk factors for recurrence and death in LSCC patients(P<0.05),and high miR-1207-5p,high miR-186-5p were protective factors(P<0.05).Conclusion:The down-regulated expression of miR-1207-5p and miR-186-5p in LSCC tissue were related to the malignant pathological characteristics of LSCC,activation of PI3K/Akt signaling pathway and low survival rate.
作者
唐一萍
冯俊
彭涛
李志勇
马鹏
罗德艳
TANG Yi-ping;FENG Jun;PENG Tao;LI Zhi-yong;MA Peng;LUO De-yan(Department ofOtolaryngology Head and Neck Surgery,The Second Clinical Medical College of Chuanbei Medical College/Nanchong Central Hospital,Nanchong,Sichuan,637000,China)
出处
《现代生物医学进展》
CAS
2023年第22期4241-4246,共6页
Progress in Modern Biomedicine
基金
四川省教育厅基金重点项目(18ZA0203)。
