摘要
为明确采自田间不同症状类型苜蓿病毒病病样中苜蓿花叶病毒(alfalfa mosaic virus,AMV)的带毒情况、症状表现与叶绿素含量的相关性及AMV的寄主范围,本试验通过田间调查采样、症状归类、丙酮乙醇混合液法和双抗体夹心酶联免疫吸附测定(DAS-ELISA)法对不同病样的叶绿素和AMV含量进行测定和检测,并对提纯至不同症状类型病样中AMV对9科32种植物的致病性进行测定。结果表明田间苜蓿病毒病病样有轻花叶、重花叶、叶片边缘褪绿黄化型和叶片畸形皱缩花叶矮化型4种症状类型,均带有AMV,且叶绿素含量、AMV含量与症状表现之间具有正相关性,症状表现最严重的叶片畸形皱缩花叶矮化型病样的叶绿素a、b、总叶绿素含量均比对照低58.00%以上,类胡萝卜素含量比对照高134.06%,AMV含量最高,为252.96 pg·mL^(-1);寄主范围测定表明AMV可侵染7科27种植物,对西葫芦致病性最强,症状表现为局部枯斑,30 d时AMV浓度为316.19 pg·mL^(-1)。
In this study,the level of alfalfa mosaic virus(AMV)and leaf chlorophyll contents in field-grown alfalfa(Medicago sativa)plants with different categories of AMV symptoms were assessed,and a test of the plant species host range of AMV was conducted.Chlorophyll content was determined by an acetone-ethanol extraction method,and AMV was quantified by a double antibody sandwich enzyme-linked immunosorbent assay(DAS-ELISA)method.The pathogenicity of AMV to 32 plant species in nine botanical families was determined for purified virus extract from plants with different symptom types.Four symptom categories of AMV disease were investigated:light mosaic,heavy mosaic,leaf edge chlorosis and yellowing and leaf deformity with leaf-wrinkling and dwarfing.There was a positive correlation between chlorophyll content and AMV content and symptom expression.For the most severe AMV symptom manifestation involving leaf deformation,wrinkling and dwarfing,the chlorophyll a,b,and total chlorophyll content was more than 58%lower than the control,and the carotenoid content was 134.06%higher than the control.The highest AMV content was 252.96 pg·mL^(-1).The host range determination showed that purified AMV could infect 27 plant species in 7 families,with the strongest pathogenicity to Cucurbita pepo.In this species,the symptoms were local withered spots,and the concentration of AMV in plant extract at 30 days after inoculation was 316.19 pg·mL^(-1).
作者
周建玲
梁巧兰
魏列新
周其宇
田龙
陈应娥
王存颖
张国印
ZHOU Jian-ling;LIANG Qiao-lan;WEI Lie-xin;ZHOU Qi-yu;TIAN Long;CHEN Ying-e;WANG Cun-ying;ZHANG Guo-yin(Biocontrol Engineering Laboratory of Crop Diseases and Pests of Gansu Province,College of Plant Protection,Gansu Agricultural University,Lanzhou 730070,China)
出处
《草业学报》
CSCD
北大核心
2024年第1期126-137,共12页
Acta Prataculturae Sinica
基金
国家自然科学基金(31860039)资助。
