摘要
以柑橘砧木枳(Poncirus trifoliata)为研究植株,以华南红壤柑橘园分离出的1株解磷细菌(phosphate solubilizing bacteria,PSB)氧化微杆菌(Microbacterium oxydans)Y8为研究菌株,通过盆栽试验探究PSB Y8对枳生长以及土壤磷素、磷循环相关功能基因的影响及其潜在的解磷机制。结果表明:接种PSB Y8极显著增加了枳的生物量以及土壤中性磷酸酶活性;显著增加了土壤中与磷活化相关的碱性磷酸酶编码基因pho D的丰度,极显著增加了葡萄糖酸编码基因gcd的丰度;显著降低了土壤中无机磷中Al-P的含量,显著增加了有机磷总含量和MLPo含量,影响了土壤中不同磷素的转化。综上,PSB Y8的解磷机制可以归纳为其通过上调磷酸酶相关功能基因pho D的丰度,增加土壤磷酸酶活性,从而影响土壤磷素组分的转化,增加土壤有机磷和MLPo的含量,可能促进枳对磷素的吸收,进而增加枳的生物量。
In this study,Poncirustrifoliataa citrus rootstock was used as the research plant,and a strain of phosphate solubilizing bacteria MicrobacteriumoxydansY8 isolated from a red soil citrus orchard in South China was studied.Pot experiment was conducted to investigate the effects of PSB Y8 on P.trifoliata growth and functional genes related to phosphorus and phosphorus cycling in soil and the potential mechanism of phosphorus removal.The results showed that inoculation of PSB Y8 very significantly increased P.trifoliatabiomass and soil neutral phosphatase activity;significantly increased the abundance of alkaline phosphatase coding gene phoD related to phosphorus activation in soil,and very significantly increased the gluconic acid coding gene gcd;significantly reduced the content of Al-P in inorganic phosphorus in the soil,and significantly increased the total organic phosphorus content and MLPo content,and affected the transformation of different phosphorus elements in the soil.To sum up,the phosphorus-resolving mechanism of PSB Y8 can be concluded that it increases soil phosphatase activity by increasing the abundance of phosphatase-related functional genes phoD,affects the conversion of soil phosphorus components,increases the content of soil organophosphorus and MLPo,and may promotes the absorption of phosphorus in poncirus aurantius,and thus increases the biomass of P.trifoliata.
作者
刘玉敏
李成江
谢小林
陈猛
周莲
王勇
朱红惠
姚青
LIU Yumin;LI Chengjiang;XIE Xiaolin;CHEN Meng;ZHOU Lian;WANG Yong;ZHU Honghui;YAO Qing(Institute of Microbiology,Guangdong Academy of Sciences,State Key Laboratory of Applied Microbiology in South China,Key Laboratory of Agricultural Microbiome and Precision Application,Ministry of Agriculture and Rural Affairs,Guangdong Provincial Key Laboratory of Bacterial Culture Preservation and Application,Guangdong Provincial Microbial Culture Preservation Center,Guangzhou 510070;Guangdong Bowoter Biotechnology Co.,Ltd.;Horticulture College of South China Agricultural University)
出处
《中国果树》
北大核心
2023年第12期73-81,共9页
China Fruits
基金
“十四五”广东省农业科技创新九大主攻方向“揭榜挂帅”项目(2023SDZG09)
2022年省级乡村振兴战略专项资金种业振兴项目(2022-440000-43010104-9463)
韶关市科技计划项目(210806164532037)
广州市科技计划项目(202201011832)。
关键词
枳
解磷细菌
磷酸酶
促生效应
磷循环
功能基因
Poncirustrifoliata
phosphate solubilizing bacteria
phosphatase
promotive effect
phosphorus cycle
functional gene
