摘要
目的观察电针通过调控基质金属蛋白酶-2(MMP-2)/金属蛋白酶组织特异性抑制剂-1(TIMP-1)对跟腱损伤大鼠生物力学及损伤修复的影响。方法将50只雄性SD大鼠按照随机数字表法分为假手术组、模型组、电针组、干扰金属蛋白酶组织特异性抑制剂-1(sh-TIMP-1)组和电针+sh-TIMP-1组,每组10只。除假手术组外,其余4组采用跟腱纵轴中间横行贯通切断法制备跟腱损伤大鼠模型。造模结束后次日,电针组大鼠取肾俞、足三里、阳陵泉,予电针治疗;sh-TIMP-1组大鼠跟腱组织中注射sh-TIMP-1质粒300μL治疗;电针+sh-TIMP-1组大鼠在跟腱组织中注射sh-TIMP-1质粒300μL,同时予电针干预,方法同电针组;假手术组、模型组大鼠不予任何干预。检测各组大鼠跟腱生物力学指标和跟腱组织病理学变化;蛋白免疫印记法检测跟腱组织中MMP-2、TIMP-1蛋白表达。结果假手术组大鼠拉断点均在肌肉连接处(跟腱无损伤缝合),评估跟腱损伤愈合情况失真,未对假手术组进行生物力学测试。电针组、sh-TIMP-1组、电针+sh-TIMP-1组大鼠最大载荷、能量吸收、弹性模量和最大应力均高于模型组(P<0.05),应变低于模型组(P<0.05);电针+sh-TIMP-1组大鼠最大载荷、能量吸收、弹性模量和最大应力均高于电针组、sh-TIMP-1组(P<0.05),应变低于电针组、sh-TIMP-1组(P<0.05)。假手术组大鼠跟腱纤维排列有序,无炎症浸润;模型组大鼠肌腱组织增生明显,纤维结构排列疏松且无序,并伴有新生血管生成和炎症细胞浸润;电针组大鼠跟腱组织得到明显改善,跟腱组织纤维结构排列较整齐,且新生血管和炎细胞浸润程度明显减轻;sh-TIMP-1组大鼠跟腱组织纤维结构排列较整齐,有新生血管和炎症细胞浸润;电针+sh-TIMP-1组大鼠跟腱组织明显改善,跟腱组织纤维结构排列较整齐,且新生血管和炎症细胞浸润程度明显减轻。与假手术组比较,模型组大鼠跟腱组织中MMP-2、TIMP-1蛋白表达及MMP-2/TIMP-1比值均升高(P<0.05);与模型组比较,电针组、sh-TIMP-1组、电针+sh-TIMP-1组大鼠跟腱组织中MMP-2、TIMP-1蛋白表达及MMP-2/TIMP-1比值均降低(P<0.05);与电针组、sh-TIMP-1组比较,电针+sh-TIMP-1组大鼠跟腱组织中MMP-2、TIMP-1蛋白表达及MMP-2/TIMP-1比值均降低(P<0.05)。结论电针可增加跟腱损伤大鼠跟腱生物力学性能,促进损伤修复,其机制与抑制MMP-2/TIMP-1表达有关。
Objective To observe the effect of electroacupuncture on biomechanics and injury repair in rats with Achilles tendon injury by regulating matrix metalloproteinase-2(MMP-2)/tissue-specific inhibitor of metalloproteinase-1(TIMP-1).Methods Fifty male Sprague-Dawley rats were randomly divided into sham group,model group,electroacupuncture group,sh-TIMP-1 group and electroacupuncture+sh-TIMP-1 group using the random number table,with 10 rats per group.The Achilles tendon injury model in rats was created by transverse transection in the middle of the longitudinal axis of the Achilles tendon.The next day after the modeling,rats in the electroacupuncture group were given electroacupuncture treatment on the Shenshu(BL23),Zusanli(ST36),and Yanglingquan(GB34).Rats in the sh-TIMP-1 group were injected with 300μL of sh-TIMP-1 into the Achilles tendon,and those in the electroacupuncture+sh-TIMP-1 group were managed by sh-TIMP-1 injection followed by electroacupuncture as above mentioned.Rats in the sham group were treated with blank control,and those in sham group and model group were not intervened.Biomechanical indicators and histopathological changes in the Achilles tendon were detected.Protein expressions of MMP-2 and TIMP-1 in the Achilles tendon tissue were detected by Western blot.Results The breaking points of the rats in the sham group were all at the muscle link,where the Achilles tendon was sutured without injury.The evaluation of Achilles tendon injury healing was distorted.No biomechanical test was performed on the sham group.The maximum load,energy absorption,elastic modulus and maximum stress of rats in the electroacupuncture group,sh-TIMP-1 group,and electroacupuncture+sh-TIMP-1 group were significantly higher than those of the model group(P<0.05),and the strain was significantly lower(P<0.05).The maximum load,energy absorption,elastic modulus and maximum stress of rats in the electroacupuncture+sh-TIMP-1 group were significantly higher than those in the electroacupuncture group and sh-TIMP-1 group(P<0.05),and the strain was significantly lower(P<0.05).In the sham group,the Achilles tendon fibers were regularly arranged without inflammatory infiltration.In the model group,the Achilles tendon tissues were significantly proliferated,and the fiber structure was loosely arranged and disordered,accompanied by neovascularization and infiltration of inflammatory cells.In the electroacupuncture group and the electroacupuncture+sh-TIMP-1 group,the tendon tissues were significantly improved,and the Achilles tendon fibers were regularly arranged,with alleviated neovascularization and infiltration of inflammatory cells.In the sh-TIMP-1 group,the Achilles tendon fibers were regularly arranged,with neovascularization and infiltration of inflammatory cells.Compared with those of sham group,the protein expressions of MMP-2 and TIMP-1,and the MMP-2/TIMP-1 ratio in the Achilles tendon tissue of rats in the model group significantly increased(P<0.05).Compared with those of the model group,the protein expressions of MMP-2 and TIMP-1,and the MMP-2/TIMP-1 ratio in the Achilles tendon tissue of rats in the electroacupuncture group,sh-TIMP-1 group and electroacupuncture+sh-TIMP-1 group were significantly lower(P<0.05).Compared with those of electroacupuncture group and sh-TIMP-1 group,the protein expressions of MMP-2 and TIMP-1,and the MMP-2/TIMP-1 ratio in the Achilles tendon tissue of rats were significantly lower in the electroacupuncture+sh-TIMP-1 group(P<0.05).Conclusion Electroacupuncture can increase the biomechanical properties of Achilles tendon in rats with Achilles tendon injury and promote injury repair by inhibiting MMP-2/TIMP-1.
作者
白文博
刘国伟
李娜
BAI Wenbo;LIU Guowei;LI Na(Department of Functional,Shijiazhuang Hospital of Traditional Chinese Medicine,Shijiazhuang,Hebei 050051;Department of Acupuncture,Shijiazhuang Hospital of Traditional Chinese Medicine,Shijiazhuang,Hebei 050051)
出处
《河北中医》
2023年第12期2032-2036,共5页
Hebei Journal of Traditional Chinese Medicine
基金
河北省中医药管理局2021年度中医药类科研指令性计划课题(编号:2021263)。
关键词
跟腱
损伤
电针疗法
动物
Achilles tendon rupture
Electroacupuncture therapy
Animal
