摘要
为探讨车前草多糖(PLP)对高脂日粮(HF)饲喂引起的小鼠脂代谢异常和氧化应激的影响,本试验选取150只SPF级雄性健康昆明小鼠,适应性饲养1周后,随机分成5个组,分别为对照(CK)组、高脂日粮(HF)组、HF+100 mg/(kg·bw)PLP组、HF+200 mg/(kg·bw)PLP组和HF+400 mg/(kg·bw)PLP组。每天记录小鼠的采食量和饮水量,试验4周后收集其肝脏和血液进行脂代谢和氧化应激相关指标和基因测定。结果显示,与CK组相比,HF组小鼠血清中甘油三脂(TG)、总胆固醇(TC)、低密度脂蛋白胆固醇(LDL-C)和血糖(GLU)含量以及天冬氨酸氨基转移酶(AST)和丙氨酸氨基转移酶(ALT)活性显著升高(P<0.05),高密度脂蛋白胆固醇(HDL-C)含量显著降低(P<0.05);肝脏中TC、TG、丙二醛(MDA)和活性氧自由基(ROS)含量显著升高(P<0.05),过氧化物酶(CAT)、谷胱甘肽过氧化物酶(GSH-Px)和超氧化物歧化酶(SOD)活性均显著降低(P<0.05)。而添加200 mg/(kg·bw)和400 mg/(kg·bw)PLP显著降低了HF饮食小鼠血清中TG、TC、LDL-C和GLU含量以及AST和ALT活性(P<0.05),显著提高HDL-C含量(P<0.05);显著降低小鼠肝脏中TC、TG、MDA和ROS含量(P<0.05);显著提高肝脏中抗氧化酶CAT、GSH-Px和SOD活性(P<0.05)。此外,进一步对小鼠抗氧化和脂代谢相关基因检测发现,PLP可显著提高HF饮食导致的抗氧化基因Nrf 2和HO-1表达水平的降低,并降低脂代谢相关基因Lp l、Cyp7αl和Scd 1的表达(P<0.05)。结果表明,HF饮食可引发小鼠脂代谢异常和肝脏氧化应激并导致肝损伤,而PLP可缓解HF饮食导致的小鼠氧化应激和脂代谢异常,该结果可为PLP的临床应用提供参考依据。
To investigate the impact of plantain polysaccharide(PLP)on lipid metabolism abnormalities and oxidative stress in mice fed with high-fat diet(HF),150 SPF male healthy Kunming mice were selected.After a week of adaptation,they were randomly divided into five groups:Control(CK)group,High-fat(HF)group,HF+100 mg/(kg·bw)PLP group,HF+200 mg/(kg·bw)PLP group,and HF+400 mg/(kg·bw)PLP group.Daily food and water intake were recorded,and after 4 weeks,liver and blood samples were collected for lipid metabolism and oxidative stress-related indicators and gene expression analysis.The results showed that compared with the CK group,mice in the HF group exhibited significantly elevated levels of serum triglycerides(TG),total cholesterol(TC),low-density lipoprotein cholesterol(LDL-C),and glucose(GLU),as well as increased aspartate transaminase(AST)and alanine transaminase(ALT)activities(P<0.05),and decreased high-density lipoprotein cholesterol(HDL-C)levels(P<0.05);liver TC,TG,malondialdehyde(MDA),and reactive oxygen species(ROS)levels were significantly increased(P<0.05),while the activities of catalase(CAT),glutathione peroxidase(GSH-Px),and superoxide dismutase(SOD)were significantly decreased(P<0.05).The addition of 200 mg/(kg·bw)and 400 mg/(kg·bw)PLP significantly decreased serum TG,TC,LDL-C,and GLU levels,as well as AST and ALT activities in HF fed mice(P<0.05),and significantly increased HDL-C levels(P<0.05),significantly reduced liver TC,TG,MDA,and ROS levels(P<0.05),while enhancing the activities of antioxidant enzymes CAT,GSH-Px,and SOD(P<0.05).Furthermore,gene expression analysis related to antioxidant and lipid metabolism in mice revealed that PLP significantly upregulated the reduced expression levels of antioxidant genes Nrf 2 and HO-1 induced by the HF diet and decreased the expression of lipid metabolism-related genes Lp l,Cyp 7α1,and Scd 1(P<0.05).These results suggest that the HF diet can induce lipid metabolism abnormalities and hepatic oxidative stress,leading to liver damage in mice.However,PLP can alleviate oxidative stress and lipid metabolism abnormalities induced by the HF diet,providing a reference for the clinical application of PLP.
作者
张力凡
朱俊义
ZHANG Li-fan;ZHU Jun-yi(Jilin Province Changbai Mountain Biological Germplasm Resources Evaluation and Application Key Laboratory,School of Life Sciences,Tonghua Normal University,Tonghua 134002,China)
出处
《中国兽医杂志》
CAS
北大核心
2023年第12期129-134,共6页
Chinese Journal of Veterinary Medicine
基金
吉林省科技发展计划项目(20210202078NC)
东北林蛙一体化设施养育技术研究与示范。
关键词
车前草多糖
高脂饮食
抗氧化酶活性
氧化应激
脂代谢
plantain polysaccharide
high-fat diet
antioxidant enzyme activity
oxidative stress
lipid metabolism
