摘要
本研究旨在探讨槐提取物和维生素C(Vitamin C,V_(C))对紫外线照射引起的HaCaT细胞光损伤的保护作用。通过体外培养HaCaT细胞并将其分为不同组别:对照组(未接受紫外辐照,无槐提取物、V_(C)或二者组合物处理)、紫外线辐射组、125μg/mL槐提取物(接受紫外辐照及槐提取物处理)、125μg/mL维生素C(接受紫外辐照及维生素C处理)以及125μg/mL组合物组(接受紫外辐照及组合物处理,槐提取物和V_(C)质量比为1:1)。采用荧光染色和酶标仪检测技术来评估槐提取物、V_(C)及二者组合物对紫外照射引起的HaCaT细胞产生的活性氧物质(ROS)和线粒体ROS的抑制率。利用免疫荧光染色和酶联免疫吸附法检测槐提取物、V_(C)和组合物对紫外照射引起的HaCaT细胞中环丁烷嘧啶二聚体(Cyclobutane pyrimidine dimers,CPDs)和细胞炎症因子IL-6的表达水平。结果显示,槐提取物、V_(C)和组合物对UVA处理后HaCaT细胞生成的总ROS抑制率分别为38.23%±9.23%、27.20%±6.87%及64.13%±4.08%,与单独使用槐提取物或V_(C)处理相比,组合物显著提高总ROS抑制率(P<0.01)。槐提取物、V_(C)和组合物对线粒体ROS(Mitochondrial DNA,mtROS)的抑制率分别为50.29%±4.92%、38.81%±8.66%及84.74%±3.68%,与单独使用槐提取物或V_(C)处理相比,组合物极显著提高mtROS抑制率(P<0.01)。此外,在UVB照射后,槐提取物、V_(C)及组合物可极显著减少CPDs的生成(P<0.01),这种效应在组合物组效果更为显著(与槐提取物组比较P<0.05;与V_(C)组比较P<0.01)。同时,槐提取物和组合物显著降低UVA照射后炎症因子IL-6的分泌(P<0.01;P<0.05),表明槐提取物及组合物具有抗炎作用。本研究结果表明槐提取物和维生素C组合物能减轻紫外线引起的氧化应激损伤和炎症反应,为未来体内研究和开发具有健康益处的槐提取物和V_(C)组合物提供实验支持。
This study aims to investigate the protective effects of the extract of Sophora japonica L.(Sophora extract)and vitamin C(V_(C))on UV-induced damage in HaCaT cells.HaCaT cells were cultured in vitro and divided into different groups:Control group(without UV irradiation or treatment with Sophora extract,V_(C),or their combination),UV radiation group,125μg/mL Sophora extract group(with UV irradiation and treatment with Sophora extract),125μg/mL vitamin C group(with UV irradiation and treatment with V_(C)),and 125μg/mL combination group(with UV irradiation,Sophora extract and V_(C)at a mass ratio of 1:1).The inhibitory effects of Sophora extract,V_(C),and their combination on reactive oxygen species(ROS)production and mitochondrial ROS in UV-irradiated HaCaT cells were measured using fluorescence staining and enzyme-linked immunosorbent assay.Immunofluorescence staining and enzyme-linked immunosorbent assay were also employed to evaluate the expression levels of cyclobutane pyrimidine dimers(CPDs)and the inflammatory cytokine IL-6 in UV-irradiated HaCaT cells treated with Sophora extract,V_(C),or their combination.The results showed that the total ROS inhibition rates of Sophora extract,V_(C),and the combination in UVA-treated HaCaT cells were 38.23%±9.23%,27.20%±6.87%,and 64.13%±4.08%,respectively.The combination significantly enhanced the total ROS inhibition rate compared to the individual treatments(P<0.01).The inhibition rates of mitochondrial ROS(mtROS)by Sophora extract,V_(C),and the combination were 50.29%±4.92%,38.81%±8.66%,and 84.74%±3.68%,respectively.The combination significantly increased the mtROS inhibition rate compared to the individual treatments(P<0.01).After UVB irradiation,the CPDs content in HaCaT cells increased,and Sophora extract,V_(C),and their combination significantly reduced CPDs formation(P<0.01),with the combination showing a more pronounced effect compared to the Sophora extract group(P<0.05)and the V_(C)group(P<0.01)alone.Furthermore,Sophora extract and the combination significantly reduced the secretion of the inflammatory cytokine IL-6 in HaCaT cells after UVA irradiation(P<0.01,P<0.05),indicating an anti-inflammatory capability.The results demonstrated that the combination of Sophora extract and Vitamin C could alleviate oxidative stress damage and inflammatory responses caused by UV radiation and provided experimental support for future in vivo studies and the development and utilization of health-promoting Sophora extract and V_(C)combinations.
作者
孙璇
李萍
孙静
郑嘉妮
余丹阳
张艳美
陈朋
SUN Xuan;LI Ping;SUN Jing;ZHENG Jiani;YU Danyang;ZHANG Yanmei;CHEN Peng(Sirio Pharma Co.,Ltd.,Shantou 515000,China;Department of Pharmacology,Shantou University Medical College,Shantou 515041,China)
出处
《食品工业科技》
CAS
北大核心
2024年第1期303-309,共7页
Science and Technology of Food Industry
