摘要
目的 研究石蒜碱对H9c2心肌细胞缺氧/复氧(H/R)损伤的保护作用及机制。方法 将H9c2心肌细胞随机分为4组:对照组、H/R组、H/R+石蒜碱低剂量组、H/R+石蒜碱高剂量组;使用CCK-8分析H9c2心肌细胞活力;用试剂盒测定乳酸脱氢酶(LDH)释放量,丙二醛(MDA)和活性氧簇(ROS)的含量,超氧化物歧化酶(SOD)和谷胱甘肽过氧化物酶(GSH-Px)活性;用流式细胞仪检测细胞凋亡率;Western blot检测Notch1蛋白水平;用Realtime聚合酶链反应检测HES-1、HES-5和Hey-1 mRNA水平。结果 与对照组比较,H/R组H9c2心肌细胞的活力减弱(P<0.05),SOD和GSH-Px的活性减弱(P<0.05),细胞凋亡率,LDH释放量,MDA和ROS含量,Notch1蛋白水平,HES-1、HES-5和Hey-1 mRNA水平均增加(P<0.05);与H/R组相比,石蒜碱低、高剂量组H9c2心肌细胞的活力增强(P<0.05),SOD和GSH-Px的活性增强(P<0.05),细胞凋亡率,LDH释放量,MDA和ROS含量均降低(P<0.05),Notch1蛋白水平,HES-1、HES-5和Hey-1 mRNA水平均增加(P<0.05)。结论 石蒜碱能减轻H/R诱导心肌细胞的损伤作用,其作用机制是否与上调Notch通路有关有待于进一步研究。
AIM To study the protective effect and mechanism of lycorine on hypoxia/reoxygenation(H/R) injury in H9c2 cardiomyocytes.METHODS H9c2 myocardial cells were randomly divided into four groups:control group,H/R group,H/R+low-dose lycorine group and H/R+high-dose lycorine group.CCK-8 was used to analyze the viability of H9c2 myocardial cells.The release of lactate dehydrogenase(LDH),the contents of malondialdehyde(MDA) and reactive oxygen species(ROS),and the activities of superoxide dismutase(SOD) and glutathione peroxidase(GSH Px) were measured with the kit.Apoptosis rate was detected by flow cytometry,Notch1 protein level was detected by Western blotting and the levels of HES-1,HES-5 and Hey-1 mRNA were detected by Real time polymerase chain reaction.RESULTS Compared with those in control group,the activity of H9c2 cardiomyocytes in H/R group decreased(P<0.05),the activity of SOD and GSH-Px decreased(P<0.05),and the apoptosis rate,LDH release,MDA and ROS content,Notch1 protein level,and HES-1,HES-5 and Hey-1 mRNA levels increased(P<0.05).Compared with those in H/R group,the activity of H9c2 cardiomyocytes in both H/R+low-dose lycorine group and H/R+high-dose lycorine group increased(P<0.05),the activity of SOD and GSH-Px increased(P<0.05),the apoptosis rate,LDH release,and MDA and ROS content decreased(P<0.05),and Notch1 protein level,HES-1,HES-5 and Hey-1 mRNA levels increased(P<0.05).CONCLUSION Lycoritine can alleviate the injury effect of H/R induced cardiomyocytes,and whether its mechanism is related to the upregulation of Notch pathway remains to be further studied.
作者
陈文明
陈嘉敏
蹇明辉
CHEN Wen-ming;CHEN Jia-min;JIAN Ming-hui(Department of Cardiovascular Medicine,Affiliated Hospital of Zunyi Medical University,Zunyi 563099,Guizhou,China;Department of Pediatrics,Affiliated Hospital of Zunyi Medical University,Zunyi 563099,Guizhou,China;Department of Respiratory and Critical Care Medicine,Affiliated Hospital of Zunyi Medical University,Zunyi 563099,Guizhou,China)
出处
《心脏杂志》
CAS
2023年第6期637-642,共6页
Chinese Heart Journal
基金
合肥医科大学附属医院硕士启动基金项目(院字2012-06)
合肥市科技支撑计划项目(遵市科合HZ字2020-227,遵市科合HZ字2021-51)。
