摘要
[目的]探索一种最适宜贝莱斯芽孢杆菌发酵的液体培养基。[方法]通过农户调研的方式,对农户自制微生物发酵液的方法进行了整理筛选,最终设计了小米粥红糖培养基(T2)、淘米水红糖培养基(T3)和红糖培养基(T4),并以LB培养基为对照培养基(T1)。将接种2%贝莱斯芽孢杆菌菌株培养原液的上述培养基制备而成的发酵液通过育苗穴盘灌溉的方式进行应用,从黄瓜幼苗生长、基质内养分含量变化和病原微生物等3个方面展开研究。[结果]T1、T2和T3处理在株高、茎粗、地上部鲜重和地上部干重4项指标上均显著优于CK处理,3个处理之间未呈现显著差异。在叶面积和叶绿素方面,T1和T3处理表现最优,2个处理之间未呈现显著差异。T4处理在株高、叶面积、地上部鲜重和地上部干重4项指标上的表现与CK处理没有显著差异,但在茎粗方面则显著优于CK处理。而在叶绿素含量方面,T4处理显著低于CK处理。T2和T4处理在总根长和总根面积2项指标上均显著优于CK处理,但与T1和T3处理之间并无显著差异。在地下部鲜重和地下部干重2项指标上,j仅T3处理的表现显著优于CK处理,其余发酵液处理在这2项指标上相对于CK处理均无显著差异,T1~T4处理在地下部干重方面同样无显著差异。利用隶属函数法和壮苗指数对黄瓜幼苗生长进行综合评价,发现T3处理对黄瓜幼苗的促生效果最为突出。在育苗后基质养分含量的变化方面,除碱解氮含量之外,T3处理在有效磷、速效钾和有机质含量方面均显著低于其余处理。通过对育苗后基质内镰刀菌属微生物的荧光定量分析发现,发酵液处理后的基质的镰刀菌属拷贝数均显著低于CK处理。[结论]10 L淘米水配合20 g红糖所制备的培养基最为适宜制备贝莱斯芽孢杆菌发酵液。
[Objective]The purpose of this study was to explore an optimal liquid medium most suitable for Bacillus velezensis fermentation.[Method]By conducting surveys among farmers,methods for the preparation of homemade microbial fermentation solution were collected and screened.Eventually,the study designed four treatments,including millet porridge with brown sugar medium(T2),rice washing water with brown sugar medium(T3),brown sugar medium(T4),and LB medium as the control treatment(T1).The above-mentioned media were prepared by inoculating 2%Bacillus velezensis strain culture mother liquor and applied through seedling tray irrigation.Research was conducted on three aspects:the growth of cucumber seedlings,changes in nutrient content in the substrate,and the types of pathogenic microorganisms.[Result]The results showed that T1,T2,and T3 treatments displayed significant improvement in four indicators,including plant height,stem thickness,aboveground fresh weight,and aboveground dry weight compared to the CK treatment,with no significant difference among the three treatments.Regarding leaf area and chlorophyll,T1 and T3 treatments performed the best,with no significant difference between the two.T4 treatment showed no significant difference in plant height,leaf area,aboveground fresh weight,and aboveground dry weight compared to CK treatment,but significantly outperformed in stem thickness.However,in chlorophyll content,T4 treatment significantly decreased compared to CK treatment.Both T2 and T4 treatments significantly outperformed the CK treatment in total root length and total root area,without significant differences between T1 and T3 treatments.Only T3 treatment significantly outperformed the CK treatment in underground fresh weight and underground dry weight,with the other fermentation treatments showing no significant difference relative to CK treatment in these two indicators.The comprehensive evaluation of cucumber seedling growth using the affiliation function method and the strength seedling index revealed that T3 treatment had the most prominent growth-promoting effect on cucumber seedlings.Regarding the changes in nutrient content in the substrate after seedling establishment,except for mineral nitrogen content,T3 treatment significantly decreased in effective phosphorus,quick-acting potassium,and organic matter content compared to other treatments.Through fluorescence quantitative analysis of Fusarium spp.in the substrates after fermentation liquid treatment,the Fusarium spp.copy numbers were significantly lower than those in the CK treatment.[Conclusion]The medium prepared with 10 L of rice washing water combined with 20 g of brown sugar was the most suitable for preparing B.velezensis fermentation broth.
作者
孙雨渤
丁祎璇
车苏桐
毛静
王文婷
汤耀中
朱秀妹
刘铮
刘欣宇
Sun Yubo;Ding Yixuan;Che Sutong;Mao Jing;Wang Wenting;Tang Yaozhong;Zhu Xiumei;Liu Zheng;Liu Xinyu(Organic Recycling Research Institute(Suzhou)of China Agricultural University,Suzhou 215100,China;College of Re-sources and Environment,China Agricultural University,Beijing 100083,China;Jiangsu Kunshan Fruit and Vegetable Science and Technology Centre,Suzhou 215300,China;College of Land Science and Technology,China Agricultural Uni-versity,Beijing 100083,China;Kunshan Yuefengdao Organic Agricultural Products Base Co.,Suzhou 215300,China;Liaoning Academy of Agricultural Sciences,Shenyang 110161,China)
出处
《山西农业大学学报（自然科学版）》
CAS
北大核心
2023年第6期10-17,共8页
Journal of Shanxi Agricultural University(Natural Science Edition)
基金
国家自然科学基金面上项目(32071552)
城乡和住房建设部科技示范项目(S20220395)
江苏省碳达峰碳中和科技创新专项(BM2022006)
辽宁省社会科学规划基金项目(L21CJY016)
辽宁省科学事业公益研究基金(软科学研究)计划项目(2022JH4/10100050)
苏州市农业科技创新项目(SNG2021012)。
关键词
调查研究
农户经验
贝莱斯芽孢杆菌
发酵液
育苗基质
Research study
Farmers'experience
Bacillus velezensis
Fermentation broth
Seedling substrate
