摘要
为探究莱阳梨膏对小鼠引咳后咳嗽的改善作用机制,采用浓氨水引咳法构建咳嗽小鼠模型,将50只小鼠随机分为5组,即空白对照组、阳性对照组(枸橼酸喷托维林)、高剂量组(12 g/kg莱阳梨膏)、中剂量组(6 g/kg莱阳梨膏)、低剂量组(3 g/kg莱阳梨膏),每组10只,共50只。每日按0.2 mL/10 g灌胃给药,为期10 d,测定咳嗽次数、潜伏期、血清超氧化物歧化酶(SOD)活性、肺脏组织过氧化物酶(POD)活性、丙二醛(MDA)含量、谷胱甘肽(GSH)含量和脾脏指数等指标。结果表明:与空白对照组相比,高剂量组降低了2 min内小鼠咳嗽次数,延长了咳嗽潜伏期,同时提升了小鼠SOD、POD活性和GSH含量,降低了MDA含量,提高了小鼠脾脏指数,差异显著(P<0.05)。中、低剂量组也表现一定改善效果,但差异不显著(P>0.05)。与阳性对照组相比,高剂量组咳嗽次数、POD活性、GSH及MDA含量、脾脏指数多个指标相近,而SOD活性超过阳性对照组,差异不显著(P>0.05)。综上,莱阳梨膏高剂量组(12 g/kg)镇咳效果优,可增强机体抗氧化能力及免疫水平。
In order to explore the mechanism of the improvement of cough in mice after taking Laiyang pear paste,a cough mouse model was established using the concentrated ammonia water cough method.The experiment was divided into a blank group,positive control group(Pentoxyverine citrate),high dose group(12 g/kg Laiyang pear paste),medium dose group(6 g/kg Laiyang pear paste),and low dose group(3 g/kg Laiyang pear paste),with 10 mice in each group and a total of 50 mice.The mice were given 0.2 mL/10 g by gavage daily for 10 days,and the number of coughs,latency period,serum SOD activity,lung tissue POD activity,MDA,GSH content and spleen index were measured.The results showed that compared with the blank control group,the high dose group significantly reduced the number of coughs in 2 minutes,prolonged the latency period of coughing,increased the SOD and POD activities and GSH content of mice,reduced the MDA content,and increased the spleen index of mice,with significant dif⁃ferences(P<0.05).The medium and low dose groups also had some improvement,but the difference was not significant(P>0.05).Compared with the posi⁃tive control group,the number of coughs,POD ac⁃tivity,GSH content,MDA content and spleen in⁃dex in the high-dose group of pear paste were similar to those in the positive control group,while SOD activity exceeded that in the positive control group without significant difference(P>0.05).In summary,The high dose group of pear paste(12 g/kg)has a superior cough suppressant effect and can effectively enhance the antioxidant capac⁃ity and immune level of the body.
作者
寇宇宁
徐登辉
林瑞麒
童陈
冯孟阳
李蕊
张佳欣
于蕾妍
KOU Yuning;XU Denghui;LIN Ruiqi;TONG Chen;FENG Mengyang;LI Rui;ZHANG Jiaxin;YU Leiyan(Haidu College,Qingdao Agricultural University,Shandong Laiyang 265200,China)
出处
《饲料工业》
北大核心
2023年第23期92-97,共6页
Feed Industry
基金
山东省教育发展促进会教育科研规划课题[JCHKT2022060]
山东省本科教改面上项目[M2022183]
海都学院青年教师校级课题[JY202107、JY202119]。
关键词
莱阳梨膏
咳嗽模型小鼠
咳嗽次数
潜伏期
抗氧化活性
Laiyang pear paste
cough model mice
cough frequency
latency period
antioxidant activity
