摘要
目的:探讨地黄饮对特应性皮炎(AD)模型小鼠的作用及机制。方法:将36只雌性BALB/c小鼠随机分为空白组、模型组、对照组、地黄饮低剂量组、地黄饮中剂量组、地黄饮高剂量组。除空白组外,其余各组均采用2,4-二硝基氯苯(2,4-dinitrochlorobenzene,DNCB)反复刺激小鼠背部皮肤诱导AD模型,对照组及地黄饮低、中、高剂量组给予相应药物灌胃,空白组及模型组小鼠予生理盐水灌胃。计算各组小鼠炎症程度评分、脏器指数(胸腺、脾脏),取背部皮损用HE染色、甲苯胺蓝染色观察组织病理学及肥大细胞变化,ELISA法检测小鼠血清总免疫球蛋白E(IgE)、干扰素-γ(IFN-γ)、白细胞介素-4(IL-4)水平,并将血清IgE、IFN-γ、IL-4水平与皮损炎症程度评分进行相关性分析。结果:与空白组比较,模型组小鼠背部皮肤出现水肿、糜烂、干燥或苔藓样改变,HE染色示小鼠背部皮肤角化不全、增厚,有炎症细胞浸润;与模型组比较,地黄饮低、中、高剂量组小鼠背部皮肤水肿、糜烂、干燥或苔藓样改变有所改善,皮肤角化不全、增厚、炎症细胞浸润情况减轻。模型组小鼠皮损炎症程度评分、肥大细胞数量、脾脏指数及血清IgE、IL-4水平高于空白组(P<0.05),血清IFN-γ水平低于空白组(P<0.05);地黄饮低、中、高剂量组皮损炎症程度评分、肥大细胞数量、脾脏指数及血清IgE、IL-4水平低于模型组(P<0.05),血清IFN-γ水平高于模型组(P<0.05)。血清IgE、IL-4水平与皮损炎症程度评分呈正相关(P<0.05);血清IFN-γ水平与皮损炎症程度评分呈负相关(P<0.05)。结论:地黄饮可能通过减少肥大细胞浸润,使免疫环境向Th2型细胞偏移的程度降低而缓解AD小鼠症状。
Objective:To investigate the effects and possible mechanisms of Dihuang decoction on atopic dermatitis(AD)model mice.Methods:Totally 36 female BALB/c mice were randomly divided into blank group,model group,control group,low-dose group of Dihuang decoction(low-dose group),medium-dose group of Dihuang decoction(medium-dose group),and high-dose group of Dihuang decoction(high-dose group).The AD model was induced by repeated stimulation of the dorsal skin with 2,4-dinitrochlorobenzene(DNCB)in all groups except the blank group.The control group,low dose group,medium dose group,and high dose group were given corresponding drugs by gavage,while the blank group and model group were given saline by gavage.Inflammation scores and organ indices(thymus and spleen)were calculated in each group,and the back skin lesions were stained with HE staining and toluidine blue staining to observe the histopathology and mast cell changes.The expression of total immunoglobulin E(IgE),interferon-γ(IFN-γ)and interleukin-4(IL-4)in the serum was detected by ELISA,and correlation analysis was carried out between the expressions of IgE,IFN-γand IL-4 and the inflammation scores in the back skin lesions.Results:Compared with the blank group,mice in the model group showed edema,erosion,dryness or moss-like changes in the appearance of the skin on the back,and HE staining showed that the skin on the back of the mice was hyperkeratotic,thickened,and infiltrated by inflammatory cells.Compared with the model group,the appearance of the skin on the back of the mice in the low,medium,and high dosage groups showed improvement in edema,erosion,dryness,or moss-like changes,and the condition of hyperkeratotic,thickened,and infiltrated by inflammatory cells of the skin was alleviated.The model group showed higher skin lesion inflammation score,mast cell number,spleen index,serum IgE,and serum IL-4 than blank group(P<0.05),while lower serum IFN-γthan blank group(P<0.05).The low,medium and high dose groups showed lower skin lesion inflammation score,mast cell number,spleen index,serum IgE and serum IL-4 than model group(P<0.05),while higher serum IFN-γthan model group(P<0.05).The serum IgE and IL-4 were positively correlated with the score of inflammation degree of skin lesions(P<0.05),while the serum IFN-γwas negatively correlated with the score of inflammation degree of skin lesions(P<0.05).Conclusion:Dihuang decoction may play a therapeutic role by alleviating symptoms in AD mice by reducing mast cell infiltration and shifting the immune environment toward Th2-type cells.
作者
林丽
马雪宁
任宇坤
杨素清
闫景东
安月鹏
LIN Li;MA Xuening;REN Yukun;YANG Suqing;YAN Jingdong;AN Yuepeng(Heilongjiang University of Chinese Medicine,Harbin Heilongjiang 150040,China;First Affiliated Hospital of Heilongjiang University of Chinese Medicine,Harbin Heilongjiang 150040,China)
出处
《中医药导报》
2023年第11期24-29,共6页
Guiding Journal of Traditional Chinese Medicine and Pharmacy
基金
黑龙江省教育厅科学技术研究项目(12511522)
哈尔滨市科技创新人才研究专项资金项目(2011RFQYS103)。
关键词
特应性皮炎
地黄饮
小鼠
作用机制
atopic dermatitis
Dihuang decoction
mice
mechanism
