摘要
目的分析贵阳市利福平耐药情况与相关突变基因特征,为控制贵阳市耐利福平结核病提供基础和依据。方法收集贵阳市公共卫生救治中心2022年6月至2023年6月疑似结核样本,采用微阵列基因芯片技术对结核分枝杆菌利福平rpoB基因进行检测,并将药敏试验验证结果于基因芯片法对比。结果3081例阳性标本中,痰标本(50.44%)及纤刷物标本(34.99%)占比最多,387例为利福平rpoB基因突变,突变率为12.84%(387/3013),6例结果显示其他突变类型,占全部突变类型的1.55%。结论贵阳市地区rpoB基因突变率为12.84%(387/3013),531位点(C→T)突变率最高,其次是511(T→C)位点、526(C→T)位点,并且与其他地区存在差异。
Objective To analysis of guiyang rifampicin with genetic mutations associated with drug resistance characteristic of rifampicin resistant TB control guiyang provide foundation and basis.Methods Suspected tuberculosis samples were collected from Guiyang Public Health Treatment Center from June 2022 to June 2023.Gene array technology was used to detect the rpoB gene of Mycobacterium tuberculosis rifampicin,and the drug sensitivity test verification results were compared with gene chip method.Results 3081 cases of positive specimens,the sputum specimens(50.44%)and the fine brush samples(34.99%)than most,387 cases of rifampicin rpoB gene mutations,mutation rate was 12.84%(387/3013),6 cases showed other mutations type,accounting for 1.55%of the total mutation types.Conclusion The mutation rate of rpoB gene in Guiyang is 12.84%(387/3013).The mutation rate of 531 site(C→T)is the highest,followed by 511 site(T→C)and 526 site(C→T),which is different from other regions.
作者
张蔓娜
陈秋悦
万亿
何云
沈渡
杨雪宇
王倩
ZHANG Manna;CHEN Qiuyue;WAN Yi;HE Yun;SHEN Du;YANG Xueyu;WANG Qian(Department of Tuberculosis,Guiyang Public Health Treatment Center,Guiyang 550003,China;Department of Infection,Guiyang Public Health Treatment Center;Department of Anesthesiology,Guiyang Public Health Treatment Center;School of Public Health and Health,Guizhou Medical University)
出处
《中国病原生物学杂志》
CSCD
北大核心
2023年第12期1470-1473,共4页
Journal of Pathogen Biology
基金
贵阳市2022年度科技计划项目(筑科合同[2022]-4-9-1号)。
关键词
结核病
结核分枝杆菌
利福平
基因芯片
tuberculosis
Mycobacterium tuberculosis
rifampin
gene chip
