摘要
目的探究柴胡皂苷A对噪音诱发耳鸣(Tinnitus)小鼠模型的干预作用及机制。方法将50只小鼠随机分为对照组(Control组)、噪音诱发耳鸣小鼠模型组(BBN组)、BBN组基础上分别灌胃10 mg/kg、20 mg/kg、40 mg/kg柴胡皂苷A(SSA)的SSA-L组、SSA-M组和SSA-H组,每组10只。检测小鼠耳鸣行为;通过旷场实验、高架十字迷宫实验和强迫游泳实验评估小鼠的抑郁样行为;ABR阈值测定小鼠听力;RTqPCR和Western blot检测小鼠听觉皮层中IL-6、TNF-α、IL-1βmRNA和蛋白表达。结果和Control组相比,BBN组小鼠在噪声暴露2 h后3 d、14 d和28 d时GPIAS%值降低(P<0.05);和BBN组相比,SSA-L组、SSAM组和SSA-H组小鼠在噪声暴露2 h后3 d、14 d和28 d时GPIAS%值升高,且呈剂量依赖(P<0.05)。和Control组相比,BBN组小鼠进入中央区时间和距离、进入开放臂次数和时间均减少,不动时间及Click和8、16、32 kHz声刺激下ABR阈值均升高(P<0.05);和BBN组相比,SSA-L组、SSA-M组和SSA-H组小鼠进入中央区时间和距离、进入开放臂次数和时间均增加,不动时间及Click和8、16、32 kHz声刺激下ABR阈值均降低(P<0.05)。和Control组相比,BBN组小鼠听觉皮层中IL-6、TNF-α、IL-1βmRNA和蛋白表达均升高(P<0.05);和BBN组相比,SSA-L组、SSA-M组和SSA-H组小鼠听觉皮层中IL-6、TNF-α、IL-1βmRNA和蛋白表达均降低(P<0.05)。结论柴胡皂苷A可改善噪声诱导耳鸣小鼠抑郁行为和听力,其可能和抑制耳鸣小鼠听觉皮层炎症因子密切相关。
Objective To investigate the intervention effect and mechanism of Saikosaponin A on noiseinduced tinnitus in a mouse model.Methods Fifty mice were randomly divided into control group(Control group),noise-induced tinnitus mouse model group(BBN group),and SSA-L group,SSA-M group and SSA-H group based on BBN group with 10 mg/kg,20 mg/kg and 40 mg/kg of Caihu saponin A by gavage,respectively,10 mice in each group.The tinnitus behavior of mice was detected;the depression-like behavior of mice was assessed by the open field experiment,elevated cross-maze experiment and forced swimming experiment;the hearing of mice was measured by ABR threshold;and IL-6,TNF-α,IL-1βmRNA and protein expression in the auditory cortex of mice were detected by RT-qPCR and Western blotting.Results Compared with the Control group,mice in the BBN group had significantly lower GPIAS%values at 3 d,14 d and 28 d after 2 h of noise exposure(P<0.05);compared with the BBN group,mice in the SSA-L,SSA-M and SSA-H groups had significantly higher GPIAS%values at 3 d,14 d and 28 d after 2 h of noise exposure in a dose-dependent manner(P<0.05).Compared with the Control group,mice in the BBN group had significantly reduced time and distance to the central zone,number and time to the open arm,and significantly higher immobility time and ABR thresholds under Click and 8,16 and 32 kHz acoustic stimulation(P<0.05);compared with the BBN group,mice in the SSA-L,SSA-M and SSA-H groups had significantly higher time and distance to the central zone,and The number and time of entering the open arm were significantly increased,and the immobility time and Click and ABR thresholds under 8,16 and 32 kHz acoustic stimulation were significantly decreased(P<0.05).Compared with the Control group,IL-6,TNF-α,IL-1βmRNA and protein expression were significantly increased in the auditory cortex of mice in the BBN group(P<0.05);compared with the BBN group,IL-6,TNF-α,IL-1βmRNA and protein expression were significantly decreased in the auditory cortex of mice in the SSA-L,SSA-M and SSA-H groups(P<0.05).Conclusion Saikosaponin A can improve depressive behavior and hearing in noise-induced tinnitus mice,which may be closely related to the inhibition of inflammatory factors in the auditory cortex of tinnitus mice.
作者
张艳
赵浩杰
段明志
段静燕
杨雪艳
ZHANG Yan;ZHAO Haojie;DUAN Mingzhi;DUAN Jingyan;YANG Xueyan(Dept.of Otolaryngology,Baoshan People's Hospital,Baoshan Yunnan 678000;Dept.of Oncology,Baoshan Yunnan 678000;Baoshan College of Traditional Chinese Medicine,Baoshan Yunnan 678000,China)
出处
《昆明医科大学学报》
CAS
2023年第11期29-37,共9页
Journal of Kunming Medical University
基金
云南省科技厅-云南中医药大学应用基础研究联合专项基金资助项目[2019FF002(-069)]。
关键词
柴胡皂苷A
耳鸣
抑郁行为
炎症反应
Saikosaponin A
Tinnitus
Depressive behavior
Inflammatory response
