摘要
建立高效液相色谱法测定化妆品中艾地苯醌的含量。化妆品样品用饱和氯化钠和甲醇涡旋、超声提取15 min后,采用OSAKA SODA CAPCELL PAK MGⅡC_(18)色谱柱(250 mm×4.6 mm,5μm)分离,柱温为40℃,以甲醇-水(80∶20)洗脱,流量为1.0 mL/min,以二极管阵列检测器进行检测,检测波长为278 nm,以色谱峰面积外标法定量分析。艾地苯醌质量浓度在1~200 mg/L范围内与色谱峰面积具有良好的线性关系,线性相关系数为0.9999,检出限为1.0 mg/kg,定量限为4.0 mg/kg。在3个添加水平下,目标物质在液体水基、膏霜、乳液、面膜、凝胶的加标平均回收率为95.61%~107.87%,测定结果的相对标准偏差为0.22%~3.07%(n=6)。该方法适用于功效化妆品中艾地苯醌的定性及定量检测。
A high performance liquid chromatography(HPLC)method was established to determine the content of idebenone in cosmetics.Cosmetic samples were extracted with saturated sodium chloride and methanol by vortex and ultrasonic extraction for 15 min.The compound was separated by OSAKA SODA CAPCELL PAK MGⅡC18 chromatographic column(250 mm×4.6 mm,5μm)with the column temperature of 40℃,and eluted with methanol-water(80∶20)as the mobile phase,the flow rate was 1.0 mL/min.The detection was performed at 278 nm by using diode array detector.The external standard method was employed for quantitative analysis.There was a good linear relationship between the mass concentration of idebenone in the range of 1-200 mg/L and the chromatographic peak area,the correlation coefficient of 0.9999.The average recoveries of the target compound in different types of cosmetics(such as water,cream,lotion,facial mask and gel)ranged from 95.61%to 107.87%at three spiked levels,with the relative standard deviations of 0.22%-3.07%(n=6).The limit of detection of this method was 1.0 mg/kg and the limit of quantification was 4.0 mg/kg.This method is suitable for the qualitative and quantitative determination of idebenone in functional cosmetics.
作者
张小媚
陈桂琴
肖树雄
ZHANG Xiaomei;CHEN Guiqin;XIAO Shuxiong(Guangdong Institute for Drug Control,NMPA Key Laboratory for Safety Risk Assessment of Cosmetics,Guangzhou 510663,China)
出处
《化学分析计量》
CAS
2023年第9期23-28,共6页
Chemical Analysis And Meterage
基金
广东省药品监督管理局科技创新项目(ZA20230014)。
