摘要
目的建立太子参Pseudostellaria heterophylla HPLC多成分定量检测方法,结合化学计量学和加权逼近理想解排序(TOPSIS)法对不同产地太子参药材质量进行评价。方法以Agilent Zorbax ODS C18柱为色谱柱,乙腈-0.2%磷酸水溶液为流动相,梯度洗脱,检测波长分别为254、285、203 nm,采用HPLC法同时检测尿苷、肌苷、鸟苷、川陈皮素、染料木苷、木犀草素、金合欢素、白杨素、水杨酸、阿魏酸、肉桂酸、太子参环肽B、太子参环肽A、乌苏酸和β-谷甾醇的含量。利用SIMCA 14.1和SPSS 26.0软件对上述15种成分含量检测结果进行主成分分析(principal component analysis,PCA)和正交偏最小二乘法-判别分析(orthogonal partial least squares-discriminant analysis,OPLS-DA),挖掘影响太子参产品质量的差异性标志物。运用加权TOPSIS法进行7省17批次太子参药材综合质量评价。结果HPLC多成分定量分析方法学考察符合《中国药典》规定要求。PCA结果显示前2个主成分特征值均大于1,累积方差贡献率为87.843%,17批次太子参样品分为3类。OPLS-DA筛选出尿苷、金合欢素、肌苷、太子参环肽A、太子参环肽B、β-谷甾醇和阿魏酸为影响太子参产品质量的差异标志物。EW-TOPSIS欧氏贴近度在0.2585~0.6425,表明不同产地太子参质量差异较大,其中江苏、安徽地区太子参排名靠前,其次为福建、江西和湖南地区,云南、四川地区太子参排名靠后。结论建立的太子参HPLC多成分定量检测方法操作便捷、结果准确可靠,PCA、OPLS-DA联合加权TOPSIS法可用于不同产地太子参药材的质量评价。
Objective To establish a HPLC multi-component quantitative detection method for Taizishen(Pseudostellariae Radix),and evaluate the quality of Pseudostellariae Radix from different producing areas by combining chemometrics and weighted TOPSIS method.Methods The separation was carried out on an Agilent Zorbax ODS C18 column using acetonitrile-0.2%phosphoric acid as the mobile phase.The detection wavelengths were set at 254,285 and 203 nm.The contents of uridine,inosine,guanosine,nobiletin,genistin,luteolin,acacetin,chrysin,salicylic acid,ferulic acid,cinnamic acid,heterophyllin B,heterophyllin A,ursolic acid andβ-sitosterol in 17 batches of Pseudostellariae Radix were determined by HPLC.Using SIMCA 14.1 and SPSS 26.0 software,principal component analysis(PCA)and orthogonal partial least squares-discriminant analysis(OPLS-DA)were performed on the detection results of the above 15 components,and the differential markers affecting the quality of Pseudostellariae Radix were excavated.The weighted TOPSIS method was used to evaluate the comprehensive quality of 17 batches of Pseudostellariae Radix from seven provinces.Results The methodological investigation of HPLC multi-component quantitative analysis met the requirements of Chinese Pharmacopoeia.PCA results showed that the eigenvalues of the first two principal components were greater than 1,and the cumulative variance contribution rate was 87.843%,and the 17 batches of Pseudostellariae Radix could be clustered into three categories.Orthogonal OPLS-DA showed that the main components that distinguish the quality of Pseudostellariae Radix were uridine,acacetin,inosine,heterophyllin A,heterophyllin B,β-sitosterol and ferulic acid.The results of EW-TOPSIS analysis showed that the average values were between 0.2585 and 0.6425,indicating that the quality of Pseudostellariae Radix from different producing areas were quite different.Pseudostellariae Radix in Jiangsu and Anhui ranked the top,followed by Fujian,Jiangxi and Hunan,and Yunnan and Sichuan ranked the bottom.Conclusion The established HPLC multi-component quantitative detection method of Pseudostellariae Radix is convenient,accurate and reliable.PCA,OPLS-DA combined with weighted TOPSIS method can be used to evaluate the quality of Pseudostellariae Radix from different habitats.
作者
李平
孙越鹏
甄会贤
程侯莲
韩晓静
冀小君
杨红
LI Ping;SUN Yue-peng;ZHEN Hui-xian;CHENG Hou-lian;HAN Xiao-jing;JI Xiao-jun;YANG Hong(Shanxi Pharmaceutical Vocational College,Taiyuan 030031,China;Tianjin Vocational College of Bioengineering,Tianjin 300301,China)
出处
《中草药》
CAS
CSCD
北大核心
2023年第17期5734-5741,共8页
Chinese Traditional and Herbal Drugs
基金
山西省教育科学“十四五”规划2021年度课题项目(GH-21421)。
