摘要
【目的】基于蜜蜂生物学及蜜蜂巢内行为研究的特点,本实验旨在研制一款新型蜜蜂巢内行为观察记录系统。【方法】在传统观察蜂箱的基础上,利用红光光源和摄像设备可对实验蜂群进行长时间、无干扰观察记录;通过在观察箱内增添滑轨、滑块、滑轮等装置,实现对具有3-5框巢脾的实验蜂群中间巢脾的观察记录。在前人设计制作的蜜蜂个体背标基础上,通过增加字母-数字-图形的排列组合,在满足肉眼易辨识的同时实现了标记样本量足够大的研究需求。【结果】成功研制了一套新型蜜蜂巢内行为学观察记录系统,将其应用于西方蜜蜂巢内行为观察实验发现:平均63.08%的被标记工蜂能够被准确识别;行为观察持续3 d后,平均43%的被标记工蜂能够被捕捉回收。【结论】实践应用表明,该系统能够较好地满足蜜蜂巢内行为学实验的各项要求,可为此类研究提供科学准确的数据。
[Objectives]To develop a new observation and recording system for studying thein-hive behavior of honeybees.[Methods]Inspired by traditional observation hives,an infrared light and camera were installed to facilitate the long-term,inobtrusive observation and recording of honeybee behavior in the hive.In addition,we added slide rails,sliders andpulleys to the hive,enabling observation and recording of the middle comb in colonies with 3-5 combs.Inspired by the individual bee markers used in previous studies,we designed a new set of markers with enlarged combinations of letters,numbers and symbols.These new,improved markers not only made it easier to identify individual bees,but also allowed us to obtain a large sample size.[Results]We successfully developed a new honeybee in-hive behavioral observation and recording system and used this to conduct in-hive behavioral observation experiments on the western honeybee,Apis melifera.We found that an average of 63.08%of tagged workers could be accurately identified,and that an average of 43%could be recaptured after 3 days of behavioral observations.[Conclusion]Results of a pilot study indicate that our new in-hive observation and recording system was an improvement on previous systems for observing in-hive honeybee behavior and data collection during behavioral experiments.
作者
葛雅婷
刘骐伟
万云星
支丹丹
周丹银
董坤
赵文正
GE Ya-Ting;LIU Qi-Wei;WAN Yun-Xing;ZHI Dan-Dan;ZHOU Dan-Yin;DONG Kun ZHAO;Wen-Zheng(Department of Apiology in Faculty of Animal Science and Techology,Yunnan Agricultural University,Kunming 650201,China)
出处
《应用昆虫学报》
CAS
CSCD
北大核心
2023年第3期835-841,共7页
Chinese Journal of Applied Entomology
基金
蜜蜂巢内行为观察蜂箱及新型个体标记的研制(2022J0289)
国家现代农业(蜂)产业技术体系(CARS-44-KXJ13)
国家自然科学基金项目(32060241)。
关键词
蜜蜂
巢内行为
个体背标
观察记录
honeybee
in-hive behavior
individual bee markers
observation and recording
