摘要
【目的】解析紫糯特种稻紫两优737及其亲本的产量、品质相关性状的基因型构成,为该品种及其双亲在育种和生产上的进一步应用提供分子依据。【方法】应用10个重要基因的13个分子标记,分析紫两优737及其亲本紫392S、福恢737在产量、品质等相关基因座位上的等位基因变化。【结果】在7个基因座位上,紫两优737及其亲本均携带相同等位基因型。在穗实粒数主效基因Gn1a座位上均携带高产等位基因Ha-Gn1a;在粒重和粒长主效基因GS3座位上均携带长粒型等位基因MH-GS3;在种皮基因Pb座位上均携带紫色果皮等位基因型;在糯稻蜡质基因wx座位上均携带低直链淀粉含量等位基因型;此外在淀粉分支酶基因SBE1和SBE3以及极限糊精酶基因PUL基因座位上亦均携带相同等位基因型。在3个基因座位上,双亲携带不同等位基因型。在Hd3a座位上,紫392S、福恢737和紫两优737分别携带Hd3a^(Nip)、hd3a^(Kasa)和Hd3a^(Nip)/hd3a^(Kasa);在Hd1座位上,分别携带Hd1^(jap)、Hd1^(ind)和Hd1^(jap)/Hd1^(ind);在焦磷酸化酶大亚基基因AGPlar座位上,分别为Ⅰ型,Ⅱ型及Ⅰ/Ⅱ杂合型。【结论】紫两优737重要农艺性状的遗传解析为其具有高产、优质、适应性好及种皮颜色等特点提供了分子证据,并为相关有利等位基因的进一步利用提供参考。
【Objective】This study was conducted to analyze the genotypes of yield and quality-related traits of the purple waxy special rice Ziliangyou 737 and its parents,and to provide molecular basis for the further application of this variety and its parents in breeding and production.【Method】Using 13 molecular markers of 10 important genes,the alleles of Ziliangyou 737 and its parents Ziliang392s and Fuhui 737 were analyzed in relation to yield and quality.【Result】In 7 gene loci,both Zizliangyou 737 and its parents carry the same allele genotype.The high-yield allele Ha-Gn1a was carried in the seat of Gn1a,the major gene of panicles number.The long grain allele MH-GS3 was carried in the GS3 locus of the major gene for grain weight and grain length.The alleles of purple pericarp were carried in the Pb locus of seed coat gene.All alleles with low amylose content in waxy gene wx locus were carried in glutinous rice.In addition,the same allelic genotype was carried in the starch branch enzyme genes SBE1 and SBE3 and the limit dextrin enzyme gene PUL gene locus.In three loci,parents carry different alleles.In the Hd3a seat,the Zi 392S,Fuhui 737 and Ziliangyou 737 carry Hd3a^(Nip),hd3a^(Kasa) and Hd3a^(Nip)/hd3a^(Kasa) respectively.On the Hd1 seat,carry Hd1^(jap),Hd1^(ind) and Hd1^(jap)/Hd1^(ind) respectively.In the large subunit gene AGPlar locus of pyrophosphorylase,there were type I,type II and hybrid type I/II,respectively.【Conclusion】The genetic analysis of important agronomic traits of Ziliangyou 737 provided molecular evidence for its high yield,high quality,good adaptability and seed coat color,and provided reference for further utilization of related favorable alleles.
作者
陈春霞
游晴如
王洪飞
郑建华
房贤涛
董瑞霞
陈双龙
廖发炼
董练飞
黄庭旭
CHEN Chunxia;YOU Qingru;WANG Hongfei;ZHENG Jianhua;FANG Xiantao;DONG Ruixia;CHEN Shuanglong;LIAO Falian;DONG Lianfei;HUANG Tingxu(Rice Research Institute,Fujian Academy of Agricultural Sciences/Fuzhou Branch,National Rice Improvement Centre of China,Fuzhou,Fujian 350018,China;Fujian Seed General Station,Fuzhou,Fujian 350001,China;Ninghua County Seed Multiplication Farm of Fujian,Ninghua,Fujian 365400,China;Agricultural and Rural Bureau of Jianyang District of Nanping City,Jianyang,Fujian 354200,China)
出处
《福建农业学报》
CAS
CSCD
北大核心
2023年第7期783-791,共9页
Fujian Journal of Agricultural Sciences
基金
福建省科技计划公益类专项(2020R1023004、2018R1021-6)
福建省科技计划重大专项(2020NZ08016)
福建省农业高质量发展超越“5511”协同创新工程项目(XTCXGC2021001)。
关键词
紫糯杂交稻
分子标记
基因分型
高产
优质
Purple waxy hybrid rice
molecular markers
genotyping
high yield
high quality
